The Usefulness of Isolated Renal Cortical Cells to Study Phosphate Transport
Much of the work on the metabolic support of transport in the kidney has utilized renal homogenates, renal slices and isolated renal tubules. However, these are not ideal systems because of their limited luminal access for transport and/or their anaerobic nature. Isolated perfused kidney and microperfused tubular segments have also been used for this purpose and do not have the above disadvantages. A useful additional tool is the use of isolated renal cells in suspension, in which access to luminal transporters and diffusion of oxygen would not be limiting.
KeywordsCortical Tubule Cytosolic Redox Renal Homogenate Renal Gluconeogenesis Zealand White Male Rabbit
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- 4.P.K. Maitra and R.W. Estabrook, A fluorometric method for the enzymic determination of glycolytic intermediates, Anal. Biochem. 7: 472 (1964).Google Scholar
- 5.M.M. Bradford, A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding, Anal. Chem. 72: 248 (1976).Google Scholar
- 9.S.R. Gullans, P.C. Brazy, V.W. Dennis, L.J. Mandel, Interaction between gluconeogenesis and sodium transport in the proximal tubule, Kidney Int. 23: 223 (1983).Google Scholar
- 13.N. Hoffman, M. Thees, R. Kinne, Phosphate transport by isolated renal brush border vesicles, Pflug. Arch. 362: 147 (1976).Google Scholar
- 14.T. Bucher and H. Sies, Mitochondrial and cytosolic redox states in perfused rat liver: Methods and problems in metabolic compartmentation in “Use of isolated liver cells and kidney tubules in metabolic studies”, Eds: J.M. Tager, H.D. Soling, J.R. Williamson. Amsterdam, p. 41 (1975).Google Scholar
- 15.N.W. Di Tullio, C.E. Berkoff, B. Blank, V, Kostos, E.J. Stack, H.L. Saunders, 3-Mercaptopicolinic acid, an inhibitor of gluconeogenesis, Biochem. J. 138: 387 (1974).Google Scholar