Advertisement

The Usefulness of Isolated Renal Cortical Cells to Study Phosphate Transport

  • Nicole Tessitore
  • Lakhi M. Sakhrani
  • Shaul G. Massry
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 178)

Abstract

Much of the work on the metabolic support of transport in the kidney has utilized renal homogenates, renal slices and isolated renal tubules. However, these are not ideal systems because of their limited luminal access for transport and/or their anaerobic nature. Isolated perfused kidney and microperfused tubular segments have also been used for this purpose and do not have the above disadvantages. A useful additional tool is the use of isolated renal cells in suspension, in which access to luminal transporters and diffusion of oxygen would not be limiting.

Keywords

Cortical Tubule Cytosolic Redox Renal Homogenate Renal Gluconeogenesis Zealand White Male Rabbit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    S.A. Kempson, A. Colon-Otero, S.L. Ou, T.P. Dousa, Possible role of nicotinamide-adenine dinucleotide (NAD) as an intra-cellular regulator of renal phosphate transport, J. Clin. Inv. 67: 1347 (1981).CrossRefGoogle Scholar
  2. 2.
    H.G. and M.E. Dew, Homogeneous cell population from rabbit kidney cortex, J. Cell. Biol. 74: 780, 1977.PubMedCrossRefGoogle Scholar
  3. 3.
    A. Vandewalle, B. Kopfer-Hobelsberger, H.G. Heidrich, Cortical cell populations from rabbit kidney isolated by free-flow electrophoresis: Characterization by measurement of hormone-sensitive adenylate cyclase, J. Cell. Biol. 92: 505 (1982).PubMedCrossRefGoogle Scholar
  4. 4.
    P.K. Maitra and R.W. Estabrook, A fluorometric method for the enzymic determination of glycolytic intermediates, Anal. Biochem. 7: 472 (1964).Google Scholar
  5. 5.
    M.M. Bradford, A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding, Anal. Chem. 72: 248 (1976).Google Scholar
  6. 6.
    R.S. Balaban, S.P. Soltoff, J.M. Storey, L.J. Mandel, Improved renal cortical tubule suspension: Spectrophotometric study of 02 delivery, Am. J. Physiol. 238: F50, (1980).PubMedGoogle Scholar
  7. 7.
    U. Schmidt and W.G. Guder, Sites of enzyme activity along the nephron, Kidney Int. 9: 233 (1976).PubMedCrossRefGoogle Scholar
  8. 8.
    A. Maleque, H. Endou, C. Koseki, F. Sakai, Nephron heterogeneity: Gluconeogenesis from pyruvate in rabbit nephron, Febs Lett. 116: 154 (1980).PubMedCrossRefGoogle Scholar
  9. 9.
    S.R. Gullans, P.C. Brazy, V.W. Dennis, L.J. Mandel, Interaction between gluconeogenesis and sodium transport in the proximal tubule, Kidney Int. 23: 223 (1983).Google Scholar
  10. 10.
    W.G. Guder and 0.H. Wieland, Metabolism of isolated kidney tubules. Additive effects of parathyroid hormone and free-fatty acids on renal gluconeogenesis, Eur. J. Biochem. 31: 69 (1972).PubMedCrossRefGoogle Scholar
  11. 11.
    K. Kurokawa and S.G. Massry, Evidence for stimulation of renal gluconeogenesis by catecholamines, J. Clin. Inv. 52: 961 (1973).CrossRefGoogle Scholar
  12. 12.
    R. Kinne, H. Murer, E. Kinne-Safran, M. Thees, G. Sachs, Sugar transport by renal plasma membrane vesicles. Characterization of the systems in the brush-broder microvilli and basal-lateral plasma membranes, J. Memb. Biol. 21: 375 (1975).CrossRefGoogle Scholar
  13. 13.
    N. Hoffman, M. Thees, R. Kinne, Phosphate transport by isolated renal brush border vesicles, Pflug. Arch. 362: 147 (1976).Google Scholar
  14. 14.
    T. Bucher and H. Sies, Mitochondrial and cytosolic redox states in perfused rat liver: Methods and problems in metabolic compartmentation in “Use of isolated liver cells and kidney tubules in metabolic studies”, Eds: J.M. Tager, H.D. Soling, J.R. Williamson. Amsterdam, p. 41 (1975).Google Scholar
  15. 15.
    N.W. Di Tullio, C.E. Berkoff, B. Blank, V, Kostos, E.J. Stack, H.L. Saunders, 3-Mercaptopicolinic acid, an inhibitor of gluconeogenesis, Biochem. J. 138: 387 (1974).Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Nicole Tessitore
    • 1
  • Lakhi M. Sakhrani
    • 1
  • Shaul G. Massry
    • 1
  1. 1.Division of NephrologyUniversity of Southern California, School of MedicineLos AngelesUSA

Personalised recommendations