Purification of γ-Aminobutyric Acid (GABA) and Benzodiazepine Receptors from Rat Brain Using Benzodiazepine-Affinity Column Chromatography
γ-Aminobutyric acid (GABA) has been established as a major inhibitory neurotransmitter in the mammalian central nervous system (1–4) as well as invertebrate nervous systems (5,6). GABA acts through a physiologically relevant receptor protein, the GABA receptor, which is labeled specifically by [3H]GABA (7) and various GABA agonists such as [3H]muscimol (8,9). On the other hand, it has been demonstrated that specific and pharmacologically relevant benzodiazepine receptors exist in the mammalian central nervous system (10,11).
KeywordsGaba Receptor Mammalian Central Nervous System Affinity Column Chromatography Sodium Thiocyanate Sodium Bicarbonate Buffer
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- 21.T. Asano, Y. Yamada, and N. Ogasawara, Soluble GABA/benzodiazepine receptors from bovine cerebral cortex, in: “Problems in GABA Research,” Y. Okada and E. Roberts, eds., Excerpta Medica, Amsterdam-Oxford-Princeton (1982).Google Scholar
- 22.Y. Ito, K. Kuriyama, E. Ueno, C. Nishimura, and Y. Yoneda, Solubilization and partial purification of cerebral GABA receptors, in: “Problems in GABA Research,” Y. Okada and E. Roberts, eds., Excerpta Medica, Amsterdam-Oxford-Princeton (1982).Google Scholar
- 26.E. Sigel, F. A. Stephenson, C. Mamalaki, and E. A Barnard, A y-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex: Purification and partial characterization, J. Biol. Chem. 258: 6965 (1983).Google Scholar
- 27.P. Cuatrecasas, Protein purification by affinity chromatography: Derivations of agarose and polyacrylamide beads, J. Biol. Chem. 245: 3059 (1970).Google Scholar
- 29.H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 183: 265 (1951).Google Scholar
- 32.K. Kuriyama and Y. Ito, Some characteristics of solubilized and partially purified cerebral GABA and benzodiazepine receptors, in: “CNS Receptors - From Molecular Pharmacology to Behavior,” P. Mandel and F. V. DeFeudis, eds., Raven Press, New York, p. 59 (1983).Google Scholar