Ca2+-Activated, Fatty Acid-Dependent Guanylate Cyclase in Synaptic Plasma Membranes and Its Requirement for Ca2+ and Mg-GTP in the Activation
Cyclic GMP in an intact cell system was reported to increase by various biologically active compounds, including muscarinic cholinergic agonists and excitatory amino acids, and supposed to act as intracellular messenger of the various neurotransmitters and hormones. Guanylate cyclase, a cyclic GMP-generating enzyme, in a cell free system can not be stimulated by agents increasing cyclic GMP contents. This suggests that the cyclase is activated by a substance generated or a process taking place within cells upon hormone stimulation. Thus cellular components such as Ca2+, fatty acids and their metabolites that are intracellularly liberated on the stimulation have been supposed to be possible physiological activators of guanylate cyclase in intact cells.
KeywordsHistamine Indomethacin Hydroperoxide Guanine Pertussis
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- 3.Asakawa, I. Scheinbaum, and R. J. Ho, Stimulation of guanylate cyclase activity by several fatty acids, Biochem. Biophys. Res. Comm. 73: 141 (1976). (1976)Google Scholar
- 4.Wallach, and I. Pastan, Stimulation of guanylate cyclase of fibroblasts by free fatty acids, J. Biol. Chem. 251: 5802 (1978).Google Scholar
- 7.G. Graff, J. H. Stephenson, D. B. Glass, M. K. Haddox, and N. D. Goldberg, Activation of soluble splenic cell guanylate cyclase by prostaglandin endoperoxides and fatty acid hydro-peroxides, J. Biol. Chem. 253: 7662 (1978).Google Scholar
- 10.D. Leiber, and S. Harbon, The relationship between the carbachol stimulatory effect on cyclic GMP content and activation by fatty acid hydroperoxides of a soluble guanylate cyclase in the guinea pig myometrium, Mol. Pharmacol. 21: 654 (1982).Google Scholar
- 15.V. P. Whittaker, I. A. Michaelson, and R. J. A. Kirkland, The separation of synaptic vesicles from nerve-ending particles (“synaptosomes”), Biochem. J. 90: 293 (1964).Google Scholar
- 18.M. Yazawa, M. Sakuma, and K. Yagi, Calmodulins from muscles of marine invertebrates, scallop and sea anemone, J. Biochem. 87: 1313 (1980).Google Scholar
- 19.R. W. Wallace, T. J. Lynch, E. A. Tallant, and W. Y. Cheung, Purification and characterization of an inhibitor protein of brain adenylate cyclase and cyclic nucleotide phosphodiesterase, J. Biol. Chem. 254: 377 (1978).Google Scholar
- 22.T. Murayama, and M. Ui, Loss of the inhibitory function of the guanine nucleotide regulatory component of adenylate cyclase due to its ADP ribosylation by islet-activating protein, pertussis toxin, in adipocyte membranes, J. Biol. Chem. 258: 3319 (1983).Google Scholar