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Biomembranes pp 193-224 | Cite as

Hydrophobic Ions, Carriers and Pore Formers in Lipid Bilayers as Studied by Fast Kinetic Methods

  • Günther Stark
Chapter
Part of the NATO ASI Series book series (NSSA, volume 76)

Abstract

Planar lipid membranes have proved as an in vitro system well suited for the study of the physical principles of ion transport through biological membranes. The black lipid films — as they are frequently called because of their “black” appearance in the reflected light — may be considered in a way as a curious phenomenon of nature. Since these structures have a thickness of only about twice the length of a lipid molecule — while extending up to several millimeters in the two other dimensions of space — one may wonder at their stable existence. It was found that a sufficient stability, i.e. sufficient lifetime of the membrane, usually depends on an adequate amount of residual solvent. According to a technique originally developed by Mueller et al.1, black lipid films may be formed by spreading a small amount of a lipid solution in an alkane solvent (e.g. decane) across a hole in a teflon septum. Montal and Mueller2 have shown that a lipid bilayer may also be obtained by apposition of two monomolecular lipid layers on the water surfaces adjacent to the two sides of the teflon septum. The membranes formed by the technique of Montal are almost solvent-free. The lifetime of membranes with a diameter exceeding a few tenths of a millimeter is, however, drastically impaired.

Keywords

Lipid Bilayer Membrane Unstirred Layer Interfacial Concentration Translocation Rate Current Relaxation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Günther Stark
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzGermany

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