Studies on the Distribution of Glycosphingolipids in Biomembranes

  • H. Wiegandt
  • K. Radsak
Chapter
Part of the NATO ASI Series book series (NSSA, volume 76)

Abstract

Among the more polar lipid constituents that contribute to the lipid bilayer of biological membranes, the glycosphingolipids show certain typical and remarkable features. Unlike the phospholipids, the glycosphingolipids occur characteristically in all animal cells concentrated at the outer leaflet of the lipid bilayer of the cellular plasma membrane. The carbohydrate moiety of the glycosphingolipids protrudes to the extracellular space, from where it may be accessible — in some cases only after trypsinisation — to other interacting molecules.

Keywords

Cholesterol Cellulose Corn Sedimentation Biodegradation 

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References

  1. 1.
    Wiegandt,H. (1982) The gangliosides, Adv. Neurochem. 4: 149–223CrossRefGoogle Scholar
  2. 2.
    Keenan,T.W., Franke,W.W., & Wiegandt,H. (1974) Ganglioside accumulation by transformed murine fibroblast (3T3) cells and canine erythrocytes, Hoppe Seyler’s Zschr. Physiol. Chem. 355: 1543–1558Google Scholar
  3. 3.
    Callies,R., Schwarzmann,G., Radsak,K., Siegert,R., & Wiegandt,H. (1977) Characterization of cellular binding of exogenous ganglioside, Eur. J. Biochem. 97: 113–118Google Scholar
  4. 4.
    Radsak,K., Schwarzmann,G., & Wiegandt,H. (1982) Studies on the cell association of exogenously added sialo-glycolipids, Hoppe-Seyler’s Zschr. Physiol. Chem. 363: 263–272Google Scholar
  5. 5.
    Wiegandt,H., Kanda,S., Inoue,K., Utsumi,K., & Nojima,Sh. (1982) Studies on the cell association of exogenous glycolipids, in New Vistas in Glycolipid Research, Adv. exptl. Med. Bio1. 152: 343–352Google Scholar
  6. 6.
    Kanda,S., Inoue,K., Nojima,Sh., Utsumi,H. & Wiegandt,H. (1982) Incorporation of a ganglioside and a spin-labelled ganglioside analogue into cell and liposomal membranes, J. Biochem. 91: 2095–2098PubMedGoogle Scholar
  7. 7.
    Yogeeswaran,G. (1981) Incorporation of asialo GM2 and gangliosides in cell surface of cultured metastatic and nonmetastatic Balb/3T3 cell lines: altered adhesion to substrate in vitro and subcutaneous tumor cell take, J. Natl. Cancer Inst. 66: 303–310PubMedGoogle Scholar
  8. 8.
    Keenan,T.W., Schmid,E., Franke,W.W., & Wiegandt,H. (1975) Exogenous glycosphingolipids suppress growth rate of transformed and untransformed 3T3 mouse cells, Expt. Cell Res. 92: 259–270Google Scholar
  9. 9.
    Icard-Liepkalns,C., Liepkalns,V.A., Yates,A.J., & Stephens,R.E. (1982) Cell cycle phases of a novel human neural cell line and the effect of exogenous gangliosides, Biochem. Biophys. Res. Com. 105: 225–230Google Scholar
  10. 10.
    Seifert,W. (1981) Gangliosides in nerve cell cultures, in Gangliosides in Neurological and Neuromuscular Function, Development, and Repair, ed. M.M. Rapport & A. Gorio, Raven Press, New York, pp 99–117Google Scholar
  11. 11.
    Dimpfel,W., Möller,W., & Mengs,U. (1981) Ganglioside–induced neurite formation in cultured neuroblastoma cells, in Gangliosides in Neurological and Neuromuscular Function, Development, and Repair, ed. M.M. Rapport and A. Gorio, Raven Press, New York, p. 119–133Google Scholar
  12. 12.
    Roisen,F.J., Bartfeld,H., & Rapport,M.M. (1981) Ganglioside mediation of in vitro neuronal maturation, in Gangliosides in Neurological and Neuromuscular Function, Development, and Repair, ed. M.M. Rapport and A. Gorio, Raven Press, New York, p. 135–150Google Scholar
  13. 13.
    Craig,S.W., & Cuatrecasa,P. (1975) Mobility of cholera toxin receptors on rat lymphocyte membranes, Proc.Natl.Acad.Sci. 72, 3844–3848CrossRefGoogle Scholar
  14. .R e vesz,T., & Greaves,A. (1975) Ligand-induced resdistribution of lymphocyte membrane ganglioside GM1, Nature 257: 10CrossRefGoogle Scholar
  15. 15.
    Sedlacek,H.H., Stärk,J., Seiler,F.R., Ziegler,W., & Wiegandt,H. (1976) Cholera toxin induced redistribution of sialoglycolipid receptor at the lymphocyte membrane, FEBS Letters 61, 272–276PubMedCrossRefGoogle Scholar
  16. 16.
    Sahyoun,N., Shatila,T., LeVine,H., & Cuatrecasas,P. (1981) Cytoskeletal association of the cholera toxin receptor in rat erythrocytes, Biochem. Biophys. Res. Com. 102: 1216–1222Google Scholar
  17. 17.
    Momoi,T., & Wiegandt,H. (1980) Separation and microdetection of oligosaccharides of glycosphingolipids by high performance cellulose thin layer chromatography-autoradiography, Hoppe-Seyler’s Zschr. Physiol. Chem. 361: 1201–1210CrossRefGoogle Scholar
  18. 18.
    Tyler,J.M., Hargreaves,W.R., & Branton,D. (1979) Purification of two spectrin-binding proteins: Biochemical and electron microscopic evidence for site-specific reassociation between spectrin and bands 2.1 and 4.1, Proc. Natl. Acad. Sci. 76, 5192–5196Google Scholar
  19. 19.
    Bennet,V., & Stenbuck,P.J. (1980) Human erythrocyte ankyrin, J. Biol. Chem. 255: 2540–2548Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • H. Wiegandt
    • 1
  • K. Radsak
    • 1
  1. 1.Dept. of Biochemistry and Dept. of Hygiene School of MedicinePhilipps-UniversityMarburgFed.Rep. Germany

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