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Phospholipid Organization in the Membranes of Normal and Abnormal Erythrocytes

  • Ben Roelofsen
  • Jos A. F. Op den Kamp
Chapter
Part of the NATO ASI Series book series (NSSA, volume 76)

Abstract

Among the various membrane systems studied so far, the erythrocyte membrane definitely is the best characterized one, particularly regarding the localization of its phospholipids. Soon after Bretscher had proposed that the phospholipids in the erythrocyte membrane might be asymmetrically distributed over both halves of the bilayer (see preceeding chapter), Zwaal and coworkers were able to complete the picture of the distribution of all of the main phospholipid classes in the human erythrocyte membrane (1,2). As a logic development, these studies have been subsequently extended to modified and pathologic erythrocytes, in attempts to gain insight into the factors which control the marked phospholipid asymmetry in this membrane.

Keywords

Erythrocyte Membrane Human Erythrocyte Membrane Beef Liver Outer Monolayer Outer Membrane Leaflet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    A.J. Verkleij, R.F.A. Zwaal, B. Roelofsen, P. Comfurius, D. Kastelijn and L.L.M. van Deenen, The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy, Biochim. Biophys. Acta 323: 178 (1973).CrossRefGoogle Scholar
  2. 2.
    R.F.A. Zwaal, B. Roelofsen, P. Confurius and L.L.M. van Deenen, Organization of phospholipids in human red cell membranes as detected by the action of various purified phospholipases, Biochim. Biophys. Acta 406: 83 (1975).CrossRefGoogle Scholar
  3. 3.
    G.van Meer, B.J.H.M. Poorthuis, K.W.A. Wirtz, J.A.F. Op den Kamp and L.L.M. van Deenen, Transbilayer distribution and mobility of phosphatidylcholine in intact erythrocyte membranes. A study with phosphatidylcholine exchange protein, Eur. J. Biochem. 103: 283 (1980).Google Scholar
  4. 4.
    W. Renooij, L.M.G. van Golde, R.F.A. Zwaal and L.L.M. van Deenen, Topological asymmetry of phospholipid metabolism in rat erythrocyte membranes, Eur. J. Biochem. 61: 53 (1976).PubMedCrossRefGoogle Scholar
  5. 5.
    W. Renooij, L.M.G. van Golde, R.F.A. Zwaal, B. Roelofsen and L.L.M. van Deenen, Preferential incorporation of fatty acids at the inside of human erythrocyte membranes, Biochim. Biophys. Acta 363: 287 (1974).CrossRefGoogle Scholar
  6. 6.
    G.V. Marinetti and R.C. Crain, Topology of amino-phospholipids in the red cell membrane, J. Supramol. Struc. 8: 191 (1978).CrossRefGoogle Scholar
  7. 7.
    A.J. Rawyler, B. Roelofsen, J.A.F. Op den Kamp and L.L.M. van Deenen, Isolation and characterization of plasma membranes from Friend erythroleukaemic cells. A study with sphingomyelinase C, Biochim. Biophys. Acta 730: 130 (1983).CrossRefGoogle Scholar
  8. 8.
    R.F.A. Zwaal and E.M. Bevers (1983), Platelet phospholipid asymmetry and its significance in hemostasis, in: “Sub-cellular Biochemistry”, Vol. 9, D.B. Roodyn, ed., Plenum Press, New York.Google Scholar
  9. 9.
    R.F.A. Zwaal, P. Confurius and L.L.M. van Deenen, Membrane asymmetry and blood coagulation, Nature 238–358 (1977).Google Scholar
  10. 10.
    C.W.M. Haest, G. Plasa, D. Kamp and B. Deuticke, Spectrin as stabiliser of the phospholipid asymmetry in the human erythrocyte membrane, Biochim. Biophys. Acta 509: 21 (1978).CrossRefGoogle Scholar
  11. 11.
    P.F.H. Franck, B. Roelofsen and J.A.F. Op den Kamp, Complete exchange of phosphatidylcholine from intact erythrocytes after protein cross-linking, Biochim. Biophys. Acta 687: 105 (1982).CrossRefGoogle Scholar
  12. 12.
    B.J.H.M. Poorthuis, J.A.F. Op den Kamp and K.W.A. Wirtz (1982), Phospholipid transfer proteins: isolation and application as membrane probes, in: “Techniques in lipid and membrane biochemistry - Part I”, B414, T.R. Hesketh, H.L. Kornberg, J.C. Metcalfe, D.H. Northcote, C.J. Pogson and K.F. Tipton, eds., Elsevier Biomedical, County Clare.Google Scholar
  13. 13.
    B. Lubin, D. Chiu, J. Bastacky, B. Roelofsen and L.L.M. van Deenen, Abnormalities in membrane phospholipid organization in sickled erythrocytes, J. Clin. Invest. 67: 1643 (1981).CrossRefGoogle Scholar
  14. 14.
    D. Chiu, B. Lubin and S.B. Shohet,Erythrocytemembrane lipid organization during the sickling process, Brit. J. Haematol. 41: 223 (1979).CrossRefGoogle Scholar
  15. 15.
    D. Chiu, B. Lubin, B. Roelofsen and L.L.M. van Deenen, Sickled erythrocytes accelerate clotting in vitro: An effect of abnormal membrane lipid asymmetry, Blood 58: 398 (1981).PubMedGoogle Scholar
  16. 15.
    D. Chiu, B. Lubin, B. Roelofsen and L.L.M. van Deenen, Sickled erythrocytes accelerate clotting in vitro: An effect of abnormal membrane lipid asymmetry, Blood 58: 398 (1981).PubMedGoogle Scholar
  17. 17.
    S.E. Lux, K.M. John and J. Karnovsky, Irreversible deformation of the spectrin-actin lattice in irreversible sickled cells, J. Clin. Invest. 58: 955 (1976).PubMedCrossRefGoogle Scholar
  18. 18.
    G. van Meer and J.A.F. Op den Kamp, Transbilayer movement of various phosphatidylcholine species in intact human erythrocytes, J. Cell. Biochem. 19: 193 (1982).PubMedCrossRefGoogle Scholar
  19. 19.
    D. Allan, A.R. Limbrick, P. Thomas and M.P. Westerman, Release of spectrin-free spicules on reoxygenation of sickled erythrocytes, Nature 295: 612 (1982).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Ben Roelofsen
    • 1
  • Jos A. F. Op den Kamp
    • 1
  1. 1.Department of BiochemistryUniversity of UtrechtUtrechtThe Netherlands

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