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Biomembranes pp 1–36Cite as

The Use of Transmission Electron Microscopy of Ultrathin Sections for the Characterization of the Ultrastructure of Normal and Damaged Bacterial Membranes

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Part of the book series: NATO ASI Series ((NSSA,volume 76))

Abstract

In the present review we shall discuss the use of some conventional techniques for the study of bacterial membranes by transmission electron microscopy of ultrathin sections.As it will be seen,such techniques,when correctly used,allow a useful analysis of bacterial membrane ultrastructure,including the characterization of the normal versus damaged membranes and the interpretation of the molecular structure of the membranes to a certain depth.By conventional electron microscopy techniques we mean fixation by aldehydes-OsO4 -uranyl or OsO4 -uranyl and embedding in the epoxy type plastics.Other less conventional procedures are available for specific purposes;details of such methods can be found in the Literature 1,2,3,4,5.

Some Methods for the Study of Bacterial Membranes by Transmission Electron Microscopy of Ultrathin Sections

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  1. Centro de Citologia Experimental da Universidade do Porto, Rua do Campo Alegre 1021, 4100, Porto, Portugal

    M. T. Silva

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  1. Burton International/Biomed, Ltd. and Harris-Stowe State College, St. Louis, Missouri, USA

    Robert M. Burton

  2. Center for Experimental Cytology, University of Porto, Porto, Portugal

    Francisco Carvalho Guerra

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Silva, M.T. (1984). The Use of Transmission Electron Microscopy of Ultrathin Sections for the Characterization of the Ultrastructure of Normal and Damaged Bacterial Membranes. In: Burton, R.M., Guerra, F.C. (eds) Biomembranes. NATO ASI Series, vol 76. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4796-5_1

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