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Hybridization of Somatic Plant Cells: Genetic Analysis

  • Yury Yu. Gleba
  • David A. Evans
Part of the Genetic Engineering book series (GEPM, volume 6)

Abstract

This review summarizes the genetic analysis of parental genetic material following fusion of somatic plant cells. The main distinction of this new technology of plant somatic hybridization is that somatic cells, not sexual cells (gametes), are used as parent material to produce hybrids. Somatic cells are treated with specific enzymes to remove their rigid cell walls, producing isolated protoplasts. The protoplasts obtained from different parental organisms can be subjected to certain experimental treatments that result in protoplast fusion. The hybrid cells produced in this way can then be grown in vitro. Hybridization by fusion of plant protoplasts is similar to hybridization of somatic animal cells. However, while only hybrid cell lines can be obtained from fused animal cells, plant protoplast fusion can be used to recover intact hybrid organisms. Hence, parasexual hybridization of plants is not only a novel way of somatic genetic analysis, but it is becoming an important tool in plant breeding.

Keywords

Large Subunit Somatic Hybrid Hybrid Plant Protoplast Fusion Fusion Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Yury Yu. Gleba
    • 1
  • David A. Evans
    • 2
  1. 1.Institute of BotanyAcademy of Science Ukrainian SSRKievUSSR
  2. 2.DNA Plant Technology CorporationCinnaminsonUSA

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