Effect of Diet on T-2 Toxicosis

  • T. K. Smith
  • M. S. Carson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 177)


T-2 toxin is an emetic trichothecene mycotoxin produced by Fusarium molds. This compound causes feed refusal, emesis and lesions in the gastrointestinal tract of livestock, poultry and man. Studies in our laboratory have indicated that the feeding of high fibre diets, non-nutritive mineral additives and high fat diets can largely overcome feed refusal caused when T-2 toxin is fed to rats. Subsequent experiments were designed to determine the mechanism by which such diets exert this effect. Rats were fed for two weeks diets containing varying levels of alfalfa meal, bentonite or corn oil in a casein-based semi-purified diet. Rats were then orally dosed with [3H] T-2 toxin and urine and feces were collected for 21 hours after which all animals were killed and tissues excised. Diet had no significant effect on the fraction dose of 3H excreted in the urine. Significant increases in fecal excretion of 3H were seen, however, with all test diets. Only high fat diets reduced hepatic residues of 3H while alfalfa had a similar effect in kidney and both alfalfa and bentonite lowered muscle residues. It was concluded that such dietary treatments overcome T-2 toxicosis mainly by promoting fecal excretion of toxin thereby reducing absorption and biological half-life.


Feed Efficiency Fecal Excretion Final Body Weight Feed Consumption Growth Trial 


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  1. Bamburg, J.R., Riggs, N.V. and Strong, F.M. (1968). The structures of toxins from two strains of Fusarium tricinctum. Tetrahedron 24, 3329 - 3336.PubMedCrossRefGoogle Scholar
  2. Carson, M.S. and Smith, T.K. (1983). Effect of feeding alfalfa and refined plant fibres on the toxicity and metabolism of T-2 toxin in rats. J. Nutr. 113: in press.Google Scholar
  3. Chadwick, R.W., Copeland, M.R. and Chadwick, C.J. (1978). Enhanced pesticide metabolism — A previously unreported effect of dietary fibre in mammals. Food Cosmet. Toxicol.16, 217–225.PubMedCrossRefGoogle Scholar
  4. Chi, M.S., Robison, T.S., Mirocha, C.J., Swanson, S.P. and Shimoda, W. (1978). Excretion and tissue distribution of radioactivity from tritium-labelled T-2 toxin in chicks. Toxicol. Appl. Pharmacol.45 391–402.PubMedCrossRefGoogle Scholar
  5. Dabich, D., Chakrapani, B. and Syner, F.N. (1968). Purification and properties of esterases characterisitic of adult rat brian. Biochem. J 110, 713–719.PubMedGoogle Scholar
  6. Ershoff, B.H. (1974). Anti-toxic effects of plant fiber. Am. J. Clin. Nutr.27, 1395–1398.PubMedGoogle Scholar
  7. Ershoff, B.H. (1977). Effects of diet on growth and survival of rats fed toxic levels of tartrazine (FD & C Yellow No. 6). J. Nutr.107, 822–828.PubMedGoogle Scholar
  8. Gefferth, G. and Blaskovits, A. (1977). The effect of different oil contents of the diet on the mixed function oxidase in the liver of rats. Nutr. Met.21 (Suppl. 1), 246–248.CrossRefGoogle Scholar
  9. George, J.R., Harbers, L.H. and Reeves, R.D. (1980). Digestive response of rats to fibre type, level and particle size. Nutr. Rep. Inter.21, 313–322.Google Scholar
  10. Guncaga, J., Lentner, C. and Haas, H.G. (1974). Determination of chromium in feces by atomic absorption spectrophotometry. Clin. Chim. Acta 57, 77–81.PubMedCrossRefGoogle Scholar
  11. Hou, I.-C., Smalley, E.B., Stron, F.M. and Ribelin, W.E. (1972). Identification of T-2 toxin in moldy corn associated with a lethal toxicosis in dairy cattle. Appl. Microbiol.24, 684–690.Google Scholar
  12. Ishii, K. and Ueno, Y. (1981). Isolation and characterization of two new trichothecenes from Fusarium Sporotrichoides strain M-l-1. Appl. Environ. Microbiol.42, 541–543.PubMedGoogle Scholar
  13. James, L.J. and Smith, T.K. (1982). Effect of alfalfa on zearalenone toxicity and metabolism in rats and swine. J. Anim. Sci.55, 110–118.PubMedGoogle Scholar
  14. Kosuri, N.R., Smalley, E.B. and Nichols, R.E. (1971). Toxicological studies of JF. Tricinctum (cordal) Synder et Hansen from moldy corn. Am. J. Vet. Res.32, 1843–1850.PubMedGoogle Scholar
  15. Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with folin phenol reagent. J. Biol. Chem.193, 265–275.PubMedGoogle Scholar
  16. Malinow, M.R., McLaughlin, P. and Stafford, C. (1980). Alfalfa seeds: effects on cholesterol metabolism. Experientia 36, 562–564.PubMedCrossRefGoogle Scholar
  17. Marasas, W.F.O., Bamburg, J.M., Smalley, E.B., Strong, F.M., Rogland, W.L. and Degurse, P.E. (1969). Toxic effects on trout, rats and mice of T-2 toxin produced by the fungus Fusarium tricinctum. Toxicol. Appl. Pharmacol.15, 471–482.PubMedCrossRefGoogle Scholar
  18. Masimango, N., Remacle, J. and Ramout, J. (1979). Elimination par des argiles conflantes de l’aflatoxine B des milieux contamines. Ann. Nutr. Aliment.33, 137–147.PubMedGoogle Scholar
  19. Matsumoto, H., Ito, T. and Ueno, Y. (1978) Toxicological approaches to the metabolites of Fusaria. XII. Fate and distribution of T-2 toxin in mice. Jjra. J. Exp. Med.48, 393–399.Google Scholar
  20. Mayes, P-A. (1977). Digestion and absorption from the gastro-intestinal tract. In: Review Of Physiological Chemistry (Harper, H.A., Rodwell, V.W. and Mayes, P.A., ed.), pp. 202–217, Lange Medical Publications, Los Altos, CA.Google Scholar
  21. Mirocha, C.J., Pathre, S.V., Schuerhamer, B. and Christensen, C.M. (1976). Natural occurrence of Fusarium toxins in feedsuffs. Appl. Environ. Microbiol. 32., 553–556.Google Scholar
  22. Myer, R.O. and Cheeke, P.R. (1975). Utilization of alfalfa meal and alfalfa protein concentrate by rats. J. Anim. Sei.40, 500–508.Google Scholar
  23. Ohta, M., Ishii, K. and Ueno, Y. (1977). Metabolism of trichothecene mycotoxins. I. Microsomal deacetylation of T-2 toxin in animal tissues. J. Biochem.82, 1591–1598.PubMedGoogle Scholar
  24. Ohta, M., Matsumoto, H., Ishii, K. and Ueno, Y. (1978). Metabolism of trichothecene mycotoxins. II. Substrate specificity of microsomal deacetylation of trichothecene. J. Biochem.84, 697–706.PubMedGoogle Scholar
  25. Puls, R. and Greenway, J.A. (1976). Fusario toxicosis from barley in B.C. II. Analysis and toxicity of suspected barley. Can. J. Comp. Med.40, 16–19.PubMedGoogle Scholar
  26. Rao, A.V. and Bright-See, E. (1979). Effect of graded amount of dietary pectin on growth parameters of rats. Nutr. Rep. Inter.19, 411–417.Google Scholar
  27. Rozman, K.K., Rozman, T.A., Williams, J. and Greim, H.A. (1982). Effect of mineral oil and/or cholestyramine in the diet on biliary and intestinal elimination of 2, 4, 5, 2f, 4’, 5f-hexabromobiphenyl in the rhesus monkey. J. Toxicol. Environ. Health 9, 611–618.PubMedCrossRefGoogle Scholar
  28. Smith, T.K. (1980a). Influence of dietary fiber, protein and zeolite on zearalenone toxicosis in rats and swine. J. Anim. Sei.50, 278–285.Google Scholar
  29. Smith, T.K. (1980b). Effect of dietary protein, alfalfa and zeolite on excretory patterns of 51, 51, 7f, 7′-[3H] zearalenone in rats. Can. J. Physiol. Pharmacol.58, 1251–1255.PubMedCrossRefGoogle Scholar
  30. Smith, T.K. (1982). Dietary influences on excretory pathways and tissue residues of zearalenone and zearalenols in the rat. Can. J. Physiol. Pharmacol. 60, in press.Google Scholar
  31. Snedecor, G.W. and Cochran, W.G. (1967). Statiscial Methods. The Iowa State University Press, Ames, IA.Google Scholar
  32. Stasse-Walthuis, M., Albers, H.F.F., Van Jeversen, J.G.C., Wil de Jong, J., Hautvast, J.G.A.J., Hermus, R.J.J., Katan, M.B., Bryden, W.G. and Eastwood, M.A. (1980). Influence of dietary fibre from vegetables and fruits, bran or citrus pectin on serum lipids, fecal lipids, and colonic function. Am. J. Clin. Nutr.33, 1745–1756.Google Scholar
  33. Story, J.A. and Kritchevsky, D. (1976). Comparison of the binding of various bile salts and bile acids in vitro by several types of fibres. J. Nutr.106, 1291–1294.Google Scholar
  34. Takeda, H. and Kiriyama, S. (1979). Correlation between the physical properties of dietary fibres and their protective activity against amaranth toxicity in rats. J. Nutr.109, 388–396.PubMedGoogle Scholar
  35. Takeda, H., Tsujita, J. and Emoto, T. (1982). Nutritional significance of dietary fiber in counteracting the amaranth- toxicity in rats: A possible explanation of the mechanism. Nutr. Rep. Inter.25, 169–187Google Scholar
  36. Ueno, Y. (1977). Trichothecenes: Overview Address. In: Mycotoxins in Human and Animal Health ( Rodricks, J,V., Hesseltine, C.W. and Mehlman, M.A., ed.), pp. 189–207, Pathotox Publishers, Inc., Park Forest South, IL.Google Scholar
  37. Vesonder, R.F., Ciegler, A., Burmeister, H.R. and Jensen, A.H. (1979). Acceptance by swine and rats of corn amended with trichothecene. Appl. Environ. Microbiol.38, 344–346.PubMedGoogle Scholar
  38. Yoshizawa, T., Swanson, S.P. and Mirocha, C.J. (1980a). In vitro metabolism of T-2 toxin in rats. Appl. Environ. Microbiol.40, 901–906.PubMedGoogle Scholar
  39. Yoshizawa, T., Swanson, S.P. and Mirocha, C.J. (1980b). T-2 metabolites in the excreta of broiler chickens administered 3H-labelled T-2 toxin. Appl. Environ. Microbiol.39, 1172–1177.PubMedGoogle Scholar
  40. Yoshizawa, T., Mirocha, C.J., Behrens, J.C. and Swanson, S.P. (1981). Metabolic fate of T-2 toxin in a lactating cow. Food Cosmet. Toxicol 19, 31–39.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • T. K. Smith
    • 1
  • M. S. Carson
    • 1
  1. 1.Department of NutritionUniversity of GuelphGuelphCanada

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