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Defenses Against Aflatoxin Carcinogenesis in Humans

  • M. Sid Masri
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 177)

Abstract

Aspects of work on aflatoxin mainly from the author’s studies are discussed, emphasizing procedures, strategies and insights related to protection against aflatoxin carcinogenesis in man due to transmission in the food chain. Five lines of defense are discussed. First, is the interfacing of the farm animal as a biologic filter between contaminated crop (feed) and human food derived from animal products. Second, is chemical detoxification of meals with ammonia and of contaminated milk by ultraviolet light. Third is prevention of mold growth and aflatoxin elaboration with ammonium carbonate for potential application during storage and drying of agricultural products. Fourth, is the development of ultrasensitive analytical methods for aflatoxins with structural proof of identity. And, fifth are natural defenses related to the role of hydroxylation of mammalian metabolism in detoxification, especially the pathway to aflatoxin Q1, a major detoxifying mechanism in primates, and the epoxidation pathway and its relation to prevention of aflatoxin residue transmission into edible tissues.

Keywords

Mold Growth Ammonium Carbonate Peanut Meal Guanine Residue Methyl Sterculate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    R. Allcroft, and R. B. A. Carnaghan, Toxic products in ground nuts. Biologic effects, Chem. and Ind. Jan. 12, 1963, p. 50.Google Scholar
  2. 2.
    K. Sargeant, Toxic products in groundnuts, chemistry and origin, Ibid, p. 53.Google Scholar
  3. 3.
    P. K. C. Austwick, and G. Ayerst, Groundnut microflora and toxicity, Ibid. p. 55.Google Scholar
  4. 4.
    A. C. Keyl, A. N. Booth, M. S. Masri, M. R. Gumbmann and W. E. Gagne, Chronic effects of aflatoxin in farm animal feeding studies, In Proc. First U.S.-Japan Conf. Toxic Microorganisms, M. Herzber, ed. (1970).Google Scholar
  5. 5.
    M. S. Masri, V. C. Garcia and J. R. Page. The aflatoxin M content of milk from cows fed known amounts of aflatoxin, Vet. Rec., 84: 146 (1969).Google Scholar
  6. 6.
    F. H. Kratzer, D. Bandy, M. Wiley and A. N. Booth, Aflatoxin effects in poultry, Proc. Soc. Exp. Biol. Med., 131: 1281 (1969).PubMedGoogle Scholar
  7. 7.
    M. R. Gumbmann, S. N. Williams, A. N. Booth, Pran Vohra, R. A. Ernst and M. Bethard, Aflatoxin susceptibility in various breeds of poultry, Ibid. 134: 683 (1970).Google Scholar
  8. 8.
    M. S. Masri, Partition of aflatoxin Bi, J. Am. Oil Chemists Soc., 47: 61 (1970).CrossRefGoogle Scholar
  9. 9.
    M. S. Masri, R. E. Lundin, J. R. Page and V. C. Garcia Crystalline aflatoxin M1 from urine and milk, Nature 215: 753 (1967).CrossRefGoogle Scholar
  10. 10.
    M. S. Masri, J. R. Page and V. C. Garcia, Analysis for aflatoxin M in milk, J. Assoc. Official Anal. Chemists, 51: 594 (1968).Google Scholar
  11. 11.
    M. S., Masri, J. R. Page and V. C. Garcia, Modification of method for aflatoxins in milk, Ibid. 52: 641 (1969).Google Scholar
  12. 12.
    M. S. Masri, H. L. E. Vix and L. A. Goldblatt, Process for detoxifying substances contaminated with aflatoxin, U.S. Patent 3, 429, 709.Google Scholar
  13. 13.
    M. S. Masri, W. F. Haddon, R. E. Lundin and D. P. H. Hsieh, Aflatoxin Q1. A newly identified major metabolite of aflatoxin B1 in monkey liver, J. Agric. Food Chem., 22: 512 (1947).CrossRefGoogle Scholar
  14. 14.
    D. P. H. Hsieh, J. I. Dalezios, R. I. Kreiger, M. S. Masri and W. F. Haddon, Use of monkey liver microsomes in production of aflatoxin Q1, Ibid. 22: 515 (1974).Google Scholar
  15. 15.
    M. S. Masri, A. N. Booth, and D. P. H. Hsieh. Comparative metabolic conversion of aflatoxin Bi to Mi and Qi. Life Sciences 15: 203 (1974).PubMedCrossRefGoogle Scholar
  16. 16.
    M. S. Masri, D. J. Hendricks and R. O. Sinnhuber, Aflatoxin Q1 major detoxifying pathway in primates, carcinogenicity tests in rainbow trout, Toxicon 17 (Suppl. 1): 116 (1979).Google Scholar
  17. 17.
    J. D. Hendricks, R. O. Sinnhuber, J. E. Nixon, J. H. Wales, M. S. Masri and D. P. H. Hsieh, Carcinogenicity of AFQ1 to rainbow trout, J. Natl. Cancer Inst., 4: 523 (1980).Google Scholar
  18. 18.
    P. Krogh, B. Hald, E. Hasselager, A. Madsen, H. P. Mortensen, A. E. Larsen and A. D. Campbell, Aflatoxin residues in bacon pigs, Pure and Appl. Chem. 35: 273 (1973).Google Scholar
  19. 19.
    R. O. Sinnhuber, D. J. Lee and J. H. Wales, Hepatic carcinogenesis of aflatoxin M1 in rainbow trout, J. Natl. Cancer Inst. 53: 1285 (1974).PubMedGoogle Scholar
  20. 20.
    R. G. Croy and G. N. Wogan, Removal of covalent aflatoxin B1 adducts from rat liver DNA, Cance Res., 4: 197 (1981).Google Scholar
  21. 21.
    P. J. Hertzog, J. R. L. Smith and R. C. Garner, Removal of DNA-bound aflatoxin B1, in rat liver and in vitro, carcinogenesis, 1: 787 (1980).Google Scholar
  22. 22.
    D. H. Swenson, J. Lin, E. C. Miller and J. A. Miller, Aflatoxin B1-2,3-oxide as a probable intermediate in the covalent binding of aflatoxin B1 and B2 to rat liver DNA and ribosomal RNA in vivo, Cancer Res., 37: 172 (1977).PubMedGoogle Scholar
  23. 23.
    D. H. Swenson, E. C. Miller and J. A. Miller, Aflatoxin B1-2,3-oxide: evidence for its formation in rat liver in vivo and by human microsomes in vitro, Biochem. Biophys. Res. Commun., 60: 1036 (1974).CrossRefGoogle Scholar
  24. 24.
    D. H. Swenson, J. A. Miller and E. C. Miller, 2,3-Dihydro-2,3-dihydroxy-aflatoxin B1: an acid hydrolysis product of an RNA-aflatoxin B1 adduct formed by hamster and rat liver microsomes in vitro, Biochem. Biophys. Res. Commun., 53: 1260 (1973).CrossRefGoogle Scholar
  25. 25.
    J. K. Lin, J. A. Miller and E. C. Miller, 2,3-Dihydro-2-(guan-7-yl)-3-hydroxy-aflatoxin B1, a major acid hydrolysis product of aflatoxin B1-ONA or ribosomal RNA adducts formed in hepatic microsome-mediated reactions and in rat liver in vivo, Cancer Res., 37: 4430 (1977).PubMedGoogle Scholar
  26. 26.
    R. G. Croy, J. M. Essigmann, V. N. Reinhold and G. N. Wogan, Identification of the principal aflatoxin B1-DNA adduct formed in vivo in rat liver, Proc. Natl. Acad. Sci. (USA), 75: 1745 (1978).CrossRefGoogle Scholar
  27. 27.
    C. N. Martin and R. C. Garner, Aflatoxin B1-oxide generated by chemical or enzymic oxidation of aflatoxin B1 causes guanine substitution in nucleic acids, Nature 267: 863 (1977).PubMedCrossRefGoogle Scholar
  28. 28.
    R. G. Croy, J. E. Nixon, R. O. Sinnhuber and G. N. Wogan, Aflatoxin B1-DNA adducts in rainbow trout embryos and liver, Carcinogenesis 1: 903 (1980).PubMedCrossRefGoogle Scholar
  29. 29.
    R. A. Bennett, J. M. Essigmann and G. N. Wogan, Excretion of aflatoxin-guanine adduct in the urine of aflatoxin B1-treated rats, Cancer Res., 41: 650 (1981).PubMedGoogle Scholar
  30. 30.
    B. F. Coles, A. M. Welch, P. J. Hertzog, J. R. Lindsay Smith and R. C. Garner, Biological and chemical studies on 8,9- dihydroxy-8,9-dihydro aflatoxin B1 and some of its esters, Carcinogenesis, 1: 79 (1980).PubMedCrossRefGoogle Scholar
  31. 31.
    L. L. Yang, V. M. Maher and M. McCormick, Error-free excision of the cytotoxic mutagenic -deoxyguanosine DNA adduct formed in human fibroblasts by 7,8-dihydroxy-9,10-epoxy- 7,8,9,10-tetrahydrobenzo [d]pyrene, Proc. Natl. Acad. Sci. USA, 77: 5933 (1980).PubMedCrossRefGoogle Scholar
  32. 32.
    W. F. Haddon, M. S. Masri, Determination of aflatoxins by high-resolution mass spectroscopy, Toxicon 17 (Suppl. 1): 64 (1979).Google Scholar
  33. 33.
    W. F. Haddon, M. S. Masri, V. Randall, R. Elsken and B. Menneghelli, J. Assoc. Official Anal. Chemists, Mass spectral analysis of aflatoxins, 60: 107 (1977).Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • M. Sid Masri
    • 1
  1. 1.Western Regional Research Center Agricultural Research ServiceU.S. Department of AgricultureAlbanyUSA

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