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The Influence of 1-(N-L-Tryptophan)-1-Deoxy-D-Fructose [FRU-TRP] and its N-Nitrosated Analogue [NO-FRU-TRP] on the Viability and Intracellular Synthetic Activity (DNA, RNA, and Protein Synthesis) of HeLa S3-Carcinoma Cells

  • D. W. Gruenwedel
  • S. C. Lynch
  • G. F. Russell
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 177)

Abstract

Exposing HeLa S3 cells at 37°C to varied concentrations of, respectively, Fru-Trp (0.1 µM – 1 mM), NO-Fru-Trp (0.1 µM – 1 mM), and NaN02 (0.6 µM – 6 mM) for varied periods of time (1–36 hr) does neither affect their viability (trypan blue dye exclusion test) nor capability to synthesize RNA or protein but is of considerable influence on DNA synthesis in the case of NO-Fru-Trp and NaN02, but not in the case of Fru-Trp which continues to be ineffective. None of the three compounds tested is of significant influence on cell number. Both NO-Fru-Trp and NaN02 stimulate DNA synthesis: a maximum of activity ([3H]thymidine incorporation) exists at the 24 hr time point of incubation, with NO-Fru-Trp, for instance, generating a 2.5-fold increase (over control) at 1 mM concentration in the medium while NaN02, at comparable concentration, increases DNA synthesis by a factor of 1.6 over control. The increase in DNA synthesis is not due to stimulatory influences on (semi-conservative) DNA replication but represents DNA repair. This was verified by keeping the cells under conditions that prevent normal (semi-conservative) replication but permit repair (“unscheduled DNA synthesis”). Two major routes are suggested by which NO-Fru-Trp could impart DNA damage and, thus, assume mutagenic properties.

Keywords

Maillard Reaction Leucine Incorporation Label Incorporation Nonenzymatic Glycosylation Amadori Compound 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • D. W. Gruenwedel
    • 1
  • S. C. Lynch
    • 1
  • G. F. Russell
    • 1
  1. 1.Department of Food Science and TechnologyUniversity of CaliforniaDavisUSA

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