Study of Cardiac Autonomic Receptors by Radiolabeled Ligand Binding Assays
The development of radioligand binding assays to study directly neurotransmitter and hormone receptors has heralded a new era of discovery of the properties and regulation of these receptors and the effectors with which they interact. In the area of cardiovascular pharmacology, application of radioligand binding techniques has facilitated significant advances in the study of receptors found in cardiac tissues, most notably the receptors of the sympathetic and parasympathetic divisions of the autonomic nervous system. β-Adrenergic receptors in cardiac muscle have been studied most extensively by these techniques, and substantial research has also been done on muscarinic cholinergic receptors. Reports of application of radioligand binding techniques to the study of cardiac α-adrenergic receptors have been less abundant, perhaps as a result of technical difficulties associated with the relatively less satisfactory available radiolabeled ligands.
KeywordsMembrane Vesicle Radioligand Binding Radioligand Binding Assay Crude Homogenate Radioligand Binding Study
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