Isolation of Cardiac Muscle Mitochondria: An Update
In cardiac muscle cells, mitochondria occupy about 40% of the total volume (Page and McCallister, 1973) and provide about 90% of the ATP required under normal aerobic conditions (Neely and Morgan, 1974). In addition to being the major source of ATP, mitochondria contain distinct Ca2+ influx and efflux systems (Carafoli, 1979), the role of which in cell function is still incompletely understood. The structure and function of mitochondria are known to be damaged in such disease conditions as myocardial ischemia (Jennings and Ganote, 1974; Rouslin, 1983; Rouslin and Millard, 1980, 1981; Sordahl and Stewart, 1980; Wood et al., 1979; Nagao et al., 1980) and heart failure (Lindenmayer et al., 1968, 1970, 1971; Lochner et al., 1968; Meerson et al., 1964; Rouslin et al., 1979; Schwartz and Lee, 1962; Wollenberger et al., 1963). A major thrust of research in this area has been the elucidation of mechanisms and factors responsible for mitochondrial damage and the design of pharmacological interventions to prevent cellular injury. In most of these studies, it is necessary to study the structure and function of mitochondria in vitro after isolation and purification.
KeywordsMitochondrial Protein Rabbit Heart Assay Medium Heart Mitochondrion Mitochondrial Pellet
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