Isolation of Canine Cardiac Sarcolemmal Vesicles
Isolation and characterization of sarcolemmal membrane fragments from the heart has been a goal of several research laboratories for some time (Wollenberger and Will, 1978). This is because of the fundamental importance of the sarcolemma in maintaining the electrophysiological and contractile properties of the heart by regulating transmembrane fluxes of the inotropically active cations Na+, K+, and Ca2+ between intracellular and extracellular compartments. Both purity and morphological form of the isolated membranes are equally important considerations in any isolation scheme for cardiac sarcolemma (Besch et al., 1976). In analogy to results first observed with cardiac sarcoplasmic reticulum membrane preparations, it seems highly advantageous to isolate sarcolemmal membrane fragments from the heart in the form of tightly sealed vesicles so that not only enzymic activity but also active and passive transport of ions can be measured in the same membrane preparation.
KeywordsATPase Activity Sarcoplasmic Reticulum Free Base Sarcolemmal Membrane Sarcoplasmic Reticulum Vesicle
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