Expression of Virus-Associated Functions in Cells Transformed in Vitro by Epstein-Barr Virus: Epstein-Barr Virus Cell Surface Antigen and Virus-Release from Transformed Cells
Epstein-Barr virus (EBV) infects resting human B lymphocytes in vitro and induces up to 10% of the infected cells to proliferate as measured in a clonal transformation assay (1–5). This virus also infects B lymphocytes in vivo; up to 20% of the peripheral B cells in patients with infectious mononucleosis (IM) express an EBV-associated nuclear antigen, EBNA (6), When peripheral lymphocytes from IM patients are collected and cloned directly in soft agar, some B cells are found to proliferate indefinitely and to express EBNA (7). Although it is not known that virus infections in vitro and in vivo are functionally equivalent, no differences between B cells infected in vitro and in vivo have yet been identified once the cells are studied in vitro. We have studied cells transformed by EBV in vitro with the hope that such studies will help to define characteristics of some of the cells infected by EBV in vivo. The advantage of studying cells transformed in vitro is that we can chart their history from their exposure to EBV to the time of study.
KeywordsCell Surface Antigen Infectious Mononucleosis Seronegative Donor 2xl04 Cell National Cancer Institute Grant
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