Advertisement

Thymosin β4

Distribution and Biosynthesis in Vertebrate Cells and Tissues
  • Bernard L. Horecker
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Thymosin β4 is one of several peptides that have been isolated from thymosin fraction 5, a preparation from calf thymus whose properties have been discussed by Low and Goldstein (Chapter 1, this volume). Following the demonstration that thymosin fraction 5 was a mixture of many peptides, ranging in molecular weight from 1000 to 14,000 and in isoelectric point from 4.0 to 7.0 (Goldstein et al., 1977; Low et al., 1979) and the elegant isolation and structural analysis of several of these peptides by Goldstein and his co-workers (Goldstein et al., 1977; Low and Goldstein, 1979; Low et al., 1981), we became interested in the mechanism of their biochemical synthesis.

Keywords

Peritoneal Macrophage Bony Fish Preimmune Serum Vertebrate Classis Thymic Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Erickson-Viitanen, S., Ruggieri, S., Natalini, P., and Horecker, B. L., 1983a, Distribution of Thymosin β4 in vertebrate classes, Arch. Biochem. Biophys. 221:570–576.PubMedCrossRefGoogle Scholar
  2. Erickson-Viitanen, S., Ruggieri, S., Natalini, P., and Horecker, B. L., 1983b, Thymosin β10, a new analogue of Thymosin ß4 in mammalian tissues, Arch. Biochem. Biophys. 225:407–413.PubMedCrossRefGoogle Scholar
  3. Lipowicz, A. W., and Horecker, B. L., 1983, In vitro synthesis of Thymosin β4 encoded by rat spleen mRNA, Proc. Natl. Acad. Sci. USA 80:1811–1815.CrossRefGoogle Scholar
  4. Freire, M., Crivellaro, O., Isaacs, C., and Horecker, B. L., 1978, Translation of mRNA from calf thymus in the wheat germ system: Evidence for a precursor of Thymosin α1, Proc. Natl. Acad. Sci. USA 75:6007–6011.PubMedCrossRefGoogle Scholar
  5. Freire, M., Hannappel, E., Rey, M., Freire, J. M, Kido, H., and Horecker, B. L., 1981, Purification of thymus mRNA coding for a 16,000 dalton polypeptide containing the thymosin α1 sequence, Proc. Natl. Acad. Sci. USA 78:192–195.PubMedCrossRefGoogle Scholar
  6. Goldstein, A. L., Low, T. L. K., McAdoo, M., McClure, J., Thurman, J. B., Rossio, J., Lai, C.-Y., Chang, D., Wang, S. S., Harvey, C., Ramel, A. H., and Meienhofer, Jr., 1977, Thymosin α1: Isolation and sequence analysis of an immunologically active thymic polypeptide, Proc. Natl. Acad. Sci. USA 74:725–729.PubMedCrossRefGoogle Scholar
  7. Goodall, G. J., Morgan, J. I., and Horecker, B. L., 1983, Thymosin β4 in cultured mammalian cell lines, Arch. Biochem. Biophys. 221:598–601.PubMedCrossRefGoogle Scholar
  8. Hannappel, E., Davoust, S., and Horecker, B. L., 1982a, Isolation of peptides from calf thymus, Biochem. Biophys. Res. Communs. 104:266–271.CrossRefGoogle Scholar
  9. Hannappel, E., Davoust, S., and Horecker, B. L., 1982b, Thymosins β8 and β9, two new peptides isolated from calf thymus homologous to Thymosin β4, Proc. Natl. Acad. Sci. USA 79:1708–1711.PubMedCrossRefGoogle Scholar
  10. Hannappel, E., Xu, G.-J., Morgan, J., Hempstead, J., and Horecker, B. L., 1982c, Thymosin β4—a ubiquitous peptide in rat and mouse tissues, Proc. Natl. Acad. Sci. 79:2172–2175.PubMedCrossRefGoogle Scholar
  11. Low, T. L. K., and Goldstein, A. L., 1979, The chemistry and biology of Thymosin II, J. Biol. Chem. 254:987–995.PubMedGoogle Scholar
  12. Low, T. L. K., Thurman, G. B., McAdoo, M., McClure, J., Rossio, J. L., Naylor, P. H., and Goldstein, A. L., 1979, The chemistry and biology of Thymosin I, J. Biol. Chem. 254:981–986.PubMedGoogle Scholar
  13. Low, T. L. K., Hu, S.-K., and Goldstein, A. L., 1981, Complete amino acid sequence of bovine thymosin β4: A thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations, Proc. Natl. Acad. Sci. USA 78:1162–1166.PubMedCrossRefGoogle Scholar
  14. Ruggieri, S., Erickson-Viitanen, S., and Horecker, B. L., 1983, Thymosin βArg10BICH, a major variant of Thymosin β10 in rabbit tissues, Arch. Biochem. Biophys. 226:388–392.PubMedCrossRefGoogle Scholar
  15. Wang, S. S., Kuleska, I. D., and Winter, D. P., 1979, Synthesis of Thymosin α1, J. Am. Chem. Soc. 101:253–254.CrossRefGoogle Scholar
  16. Xu, G.-J., Hannappel, E., Morgan, J., Hempstead, J., and Horecker, B. L., 1982, Synthesis of “Thymosin” β4 by peritoneal macrophages and adherent spleen cells, Proc. Natl. Acad. Sci. USA 79:4006–4009.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Bernard L. Horecker
    • 1
  1. 1.Roche Institute of Molecular BiologyRoche Research CenterNutleyUSA

Personalised recommendations