Advertisement

Interleukin-3 Production and Action in Tumor-Bearing Hosts

  • Carol J. Burger
  • Klaus D. Elgert
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Examination of the differentiation pathway leading to clonal expansion of cytotoxic T lymphocytes (CTL) has been approached by many methods (Cantor and Boyse, 1975; Gillis et al., 1979; Reinherz et al., 1980). Detection of soluble factors that provide proliferative signals has afforded a new tool for dissecting this pathway. The proposed sequential cascade model, resulting in CTL maturation, begins with the interaction of macrophage (Mφ)-derived interleukin-1 (IL-1) with antigen- or mitogen-primed helper T (Th) cells (Farrar et al., 1980). Th cells then produce both IL-2 (Hancock et al., 1981) and IL-3 (Ihle et al., 1981a). IL-2 stimulates CTL precursor proliferation and immune interferon production which leads to the production of specific, mature CTL (Farrar et al., 1981).

Keywords

Fresh Cell Splenic Lymphocyte Fibrosarcoma Cell Normal Host Nylon Wool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bonnard, G. D., Yasaka, K., and Jacobson, D., 1979, Ligand-activated T cell growth factor-induced proliferation: Absorption of T cell growth factor by activated T cells, J. Immunol. 123:2704.PubMedGoogle Scholar
  2. Burger, C. J., Elgert, K. D., and Farrar, W. L., 1984, Interleukin 2 (IL-2) activity during tumor growth: IL-2 production kinetics, absorption of and responses to exogenous IL-2, Cell. Immunol. 84 (in press).Google Scholar
  3. Cantor, H., and Boyse, E. A., 1975, Functional subclasses of T lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen, J. Exp. Med. 141:1376PubMedCrossRefGoogle Scholar
  4. Elgert, K. D., and Connolly, K. M., 1978, Macrophage regulation of the T cell allogeneic response during tumor growth, Cell. Immunol. 35:1.PubMedCrossRefGoogle Scholar
  5. Elgert, K. D., and Farrar, W. L., 1978, Suppressor cell activity in tumor-bearing mice. I. Dualistic inhibition by suppressor T lymphocytes and macrophages, J. Immunol. 120:1345.PubMedGoogle Scholar
  6. Enjuanes, L., Lee, J. C., and Ihle, J. N., 1981, T cell recognition of Moloney sarcoma virus proteins during tumor regression. I. Lack of a requirement for macrophages and the role of blastogenic factors in T cell proliferation, J. Immunol. 126:1478.PubMedGoogle Scholar
  7. Farrar, W. L., and Elgert, K. D., 1978a, Inhibition of mitogen and immune blastogenesis by two distinct populations of suppressor cells present in the spleen of fibrosarcoma-bearing mice: Adoptive transfer of suppression, Int. J. Cancer 22:142.PubMedCrossRefGoogle Scholar
  8. Farrar, W. L., and Elgert, K. D., 1978b, In vitro immune blastogenesis during contact sensitivity in tumor-bearing mice. II. Mechanisms of inhibition, Cell. Immunol. 40:365.PubMedCrossRefGoogle Scholar
  9. Farrar, W. L., Mizel, S. B., and Farrar, J. J., 1980, Participation of lymphocyte activating factor (interleukin 1) in the induction of cytotoxic T cell responses, J. Immunol. 124:1371.PubMedGoogle Scholar
  10. Farrar, W. L., Johnson, H. M., and Farrar, J. J., 1981, Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2, J. Immunol. 126:1120.PubMedGoogle Scholar
  11. Gillis, S., Union, N. A., Baker, P. E., and Smith, K. A., 1979, The in vitro generation and sustained culture of nude mouse cytolytic T-lymphocytes, J. Exp. Med. 149:1460.PubMedCrossRefGoogle Scholar
  12. Hancock, E. J., Kilburn, D. G., and Levy, J. B., 1981, Helper cells active in the generation of cytotoxicity to a syngeneic tumor, J. Immunol. 127:1394.PubMedGoogle Scholar
  13. Itile, J. N., Lee, J. C., and Rebar, L., 1981a, T cell recognition of Moloney leukemia virus proteins. III. T cell proliferative responses against gp70 are associated with the production of a lymphokine inducing 20-alpha-hydroxysteriod dehydrogenase on splenic lymphocytes, J. Immunol. 127:2565.Google Scholar
  14. Ihle, J. N., Peppersack, L., and Rebar, L., 1981b, Regulation of T cell differentiation: In vitro induction of 20α-hydroxysteriod dehydrogenase in splenic lymphocytes from athymic mice by a unique lymphokine, J. Immunol. 126:2184.PubMedGoogle Scholar
  15. Ihle, J. N., Hapel, A., Greenberg, J., Lee, J. C., and Rein, A., 1982, Possible roles of interleukin 3 in the regulation of lymphocyte differentiation, in: The Potential Role of T Cells in Cancer Therapy (A. Fefer and A. Goldstein, eds.), pp. 93–112, Raven Press, New York.Google Scholar
  16. Mills, G. B., and Paetkau, V., 1980, Generation of cytotoxic lymphocytes to syngeneic tumor using co-stimulator (interleukin 2), J. Immunol. 125:1897.PubMedGoogle Scholar
  17. Reinherz, E. L., Moretta, L., Roper, M., Breard, J. M., Mingari, M. C., Cooper, M. D., and Schlossman, S. F., 1980, Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies: A comparison, J. Exp. Med. 151:969.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Carol J. Burger
    • 1
  • Klaus D. Elgert
    • 1
  1. 1.Department of Biology, Microbiology SectionVirginia Polytechnic Institute and State UniversityBlacksburgUSA

Personalised recommendations