Advertisement

Expression of T-Cell Markers on Chicken Bone Marrow Precursor Cells Incubated with an Avian Thymic Hormone

  • Krishna K. Murthy
  • Frances G. Beach
  • William L. Ragland
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Thymic extracts from a number of mammals (mice, rats, calves, pigs, and humans) have been shown to contain a variety of hormones or factors which play an important role in the differentiation and maturation of T lymphocytes. Some of the well-characterized hormones or factors include thymosin (Goldstein, 1976), thymulin (Bach et al., 1978), thymopoietin (Goldstein, 1975), and thymic humoral factor (Kook et al., 1975). These and several additional factors have also been demonstrated to be present in the serum of some of the mammals. Most of these hormones are active in bioassays both in vitro and in vivo. Thymosin fraction 5 induces the expression of Thy-1 and Lyt antigens on murine precursor cells and functional maturation of T cells (Ahmed et al., 1978). It also has been shown to induce HTLA surface marker on human bone marrow cells (Touraine et al., 1975) and to enhance the number of E-rosette-forming T cells (Incefy et al., 1975). Similar biological activity has been reported for thymulin (Bach et al., 1978). Mouse bone marrow cells or spleen cells express TL and Thy-1 antigens when incubated in vitro with thymopoietin (Basch and Goldstein, 1974).

Keywords

Bone Marrow Cell Precursor Cell Indirect Fluorescent Antibody Test Indirect Fluorescent Antibody Mouse Bone Marrow Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahmed, A., Smith, A. H., Wong, D. M., Thurman, G. B., Goldstein, A. L., and Sell, K. W., 1978, In vitro induction of Lyt surface markers on precursor cells incubated with thymosin polypeptides, Cancer Treat. Rep. 62:1739–1747.PubMedGoogle Scholar
  2. Bach, J.-F., Bach, M.-A., Charreire, J., Dardenne, M., and Pléau, J. M., 1978, The mode of action of thymic hormones, Ann. N.Y. Acad. Sci. 332:23–32.CrossRefGoogle Scholar
  3. Barger, B. O., Pace, J. L., Inman, F. P., and Ragland, W. L., 1977, The purification and physico-chemical characterization of a chicken thymus specific antigen, Fed. Proc. 36:1237.Google Scholar
  4. Basch, R. S., and Goldstein, G., 1974, Induction of T-cell differentiation in vitro by thymin, a purified polypeptide hormone of the thymus, Proc. Natl. Acad. Sci. USA 71:1474–1478.PubMedCrossRefGoogle Scholar
  5. Goldstein, A. L., 1976, The history of the development of thymosin: Chemistry, biology and clinical applications, Trans. Am. Clin. Climatol. Soc. 88:79–94.Google Scholar
  6. Goldstein, G., 1975, The isolation of thymopoietin (thymin), Ann. N.Y. Acad. Sci. 249:177–185.PubMedCrossRefGoogle Scholar
  7. Hudson, L., and Roitt, I. M., 1973, Immunofluorescent detection of surface antigens specific for B and T lymphocytes in the chicken, Eur. J. Immunol. 3:63–67.PubMedCrossRefGoogle Scholar
  8. Incefy, G. S., L’esperance, P., and Good, R. A., 1975, In vitro differentiation of human marrow cells into T lymphocytes by thymic extracts using the rosette technique, Clin. Exp. Immunol. 19:475–483.PubMedGoogle Scholar
  9. Komuro, K., and Boyse, E. A., 1973, Induction of T lymphocytes from precursor cells in vitro by a product of the thymus, J. Exp. Med. 138:479–482.PubMedCrossRefGoogle Scholar
  10. Komuro, K., Goldstein, G., and Boyse, E. A., 1975, Thymus-repopulating capacity of cells that can be induced to differentiate to T cells in vitro, J. Immunol. 115:195–198.Google Scholar
  11. Kook, A. I., Yakir, Y., and Trainin, N., 1975, Isolation and partial chemical characterization of THF, a thymus hormone involved in immune maturation of lymphoid cells, Cell. Immunol. 19:151–157.PubMedCrossRefGoogle Scholar
  12. Miller, H. C., Schmiege, S. K., and Rule, A., 1973, Production of functional T cells after treatment of bone marrow with thymic factor, J. Immunol. 111:1005–1009.PubMedGoogle Scholar
  13. Murthy, K. K., Odend’hal, S., and Ragland, W. L., 1984, Demonstration of T lymphocytes in the bursa of Fabricius of the chicken following cyclophosphamide treatment, Dev. Comp. Immunol. 8: 213–218.PubMedCrossRefGoogle Scholar
  14. Pace, J. L., Barger, B. O., Dawe, D. L., and Ragland, W. L., 1978, Specific antigens of chicken thymus, Eur. J. Immunol. 8:671–678.PubMedCrossRefGoogle Scholar
  15. Reisner, Y., Itzicovitch, L., Meshorer, A., and Sharon, N., 1978, Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins, Proc. Natl. Acad. Sci. USA 75:2933–2936.PubMedCrossRefGoogle Scholar
  16. Touraine, J. L., Touraine, F., Incefy, G. S., and Good, R. A., 1975, Effect of thymic factors on the differentiation of human marrow cells into T-lymphocytes in vitro in normals and patients with immunodeficiencies, Ann. N.Y. Acad. Sci. 249:335–342.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Krishna K. Murthy
    • 1
  • Frances G. Beach
    • 1
  • William L. Ragland
    • 1
  1. 1.Poultry Disease Research Center, Department of Avian Medicine, College of Veterinary MedicineUniversity of GeorgiaAthensUSA

Personalised recommendations