Augmentation of Antibody Synthesis in Vitro by Thymosin Fraction 5
Previously, techniques available for the measurement of specific antibody synthesized in vitro have been complex and have usually required the addition of a nonspecific mitogen, such as pokeweed mitogen (PWM) (Fauci and Pratt, 1976; Thomson and Harris, 1977; Stevens and Saxon, 1978). We have recently described a one-step, non-mitogen-requiring assay for measuring antibody production in vitro (Ershler et al., 1982; Moore et al., 1984). One advantage of this assay is that with the addition to the culture medium of biological response modifiers such as thymosin or lymphokines, it allows the evaluation of these agents on this important immunological function. In this chapter, the effect of thymosin fraction 5 (TF5) on specific antitetanus and anti-influenza antibody production will be discussed. The in vitro effect of TF5 was found to vary with age, the most profound effect being observed in the advanced age groups (Ershler et al., 1984b). The potential therapeutic implications of these observations will also be discussed.
KeywordsAntibody Production Tetanus Toxoid Biological Response Modifier Booster Immunization Pokeweed Mitogen
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