Silicic Acid Batch Adsorption Procedure for the Partial Purification of Interleukin-2 from Cultures of Primary Lymphocytes and Long-Term T-Cell Lines
Silicic acid (SA; approximately H2SiO3 or as hydrated silica, SiO·H2O) has been used by many investigators as a solid matrix for adsorption chromatography. This subject has been reviewed by Huaser (1955) and Unger (1979). Particularly noteworthy is the utilization of SA for the separation of natural lipid mixtures and highly polar complexes (Hirsch and Ahrens, 1958; Mangold and Malins, 1960). SA possesses a surface that can have widely varying properties because of the random distribution of different groups, including: (1) silanol groups (-Si-OH), (2) siloxan groups (-Si-O-Si), and (3) hydrated silanol groups (-Si-OH·H2O) (Smith, 1969). As with silica gel (SiO2), the adsorptive activity of SA is due primarily to surface silanol groups, and these sites are hydrogen-binding, weakly acidic, and therefore polar (Freeman, 1982). A reaction of silica with chlorosilanes results in the formation of siloxane-type phase matrices that are widely used in reverse-phase high-performance liquid chromatography (rpHPLC) for protein isolation and analysis (Voelter, 1981).
KeywordsSilicic Acid Partial Purification Adsorption Chromatography Surface Silanol Group Hydrated Silica
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- Hauser, E. A., 1955, Silicic Sciences, Van Nostrand, Princeton, New Jersey.Google Scholar
- Pauly, J. L., Twist, C. J., Pirela, D. L., Reinertson, R. R., Callahan, J. J., Jr., and Russell, C. W., 1982b, Studies of cultured human T lymphocytes. III. A quantiative assay of the human T cell growth-promoting lymphokine interleukin-2, IRCS Med. Sci. Biochem. 10:899–900.Google Scholar
- Pauly, J. L., Twist, C. J., Pirela, D. L., and Russell, C. W., 1983, Studies of cultured human T lymphocytes. V. Isolation and partial purification of the human T cell growth-promoter interleukin-2 by adsorption onto silicic acid, IRCS Med. Sci. Biochem. 11:184–185.Google Scholar
- Smith, I., 1969, Chromatographic and Electrophoretic Techniques, 3rd ed., Vol. 1, pp. 457–493, Wiley, New York.Google Scholar
- Unger, K. K., 1979, Porous Silica, Elsevier, Amsterdam.Google Scholar
- Voelter, W., 1981, High performance liquid chromatography in peptide research, in: High Performance Liquid Chromatography in Protein and Peptide Chemistry (F. Lottspeich, A. Henschen, and K. P. Hupe, eds.), pp. 1–53, de Gruyter, Berlin.Google Scholar