Lymphotoxins, a Multicomponent Family of Effector Molecules

Purification of Human α-Lymphotoxin from a Cloned Continuous Lymphoblastoid Cell Line IR 3.4 to Homogeneity
  • Gale A. Granger
  • Diane L. Johnson
  • J. Michael Plunkett
  • Irene K. Masunaka
  • Sally L. Orr
  • Robert S. Yamamoto
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Human lymphotoxins (LT) are a multicomponent family of related and distinct cell-lytic and growth inhibitory glycoproteins (Toth and Granger, 1979). They are inducible and can be released specifically when immune lymphoid cells are cocul-tured with the immunizing antigen or nonspecifically when nonimmune lymphoid cells are cocultured with lectins (Daynes and Granger, 1974; Granger and Williams, 1968; Granger et al., 1969). An exception to the inducible mode of release of these effector molecules is that certain continuous human lymphoblastoid cell lines con-stitutively release low levels of LT in vitro (Fair et al., 1979; Granger et al., 1970; Khan et al., 1982). There is now evidence that certain LT forms may have a role as lytic effectors in different classes of cell destructive reactions mediated by human lymphocytes in vitro (Weitzen et al., 1983a,b; Yamamoto et al., 1979, 1984b). However, the situation is complex for it is clear that different classes of effector lymphocytes (T cells, NK cells) can release different LT forms (Harris et al., 1981; Weitzen et al., 1983a,b; Wright and Bonavida, 1982; Yamamoto et al., 1984a). Moreover, in these reactions the different LT forms may assemble together to induce cell lysis as molecular complexes or alternatively may act as individual components on a target cell in a synergistic fashion.


Phorbol Myristate Acetate Lytic Activity Phorbol Myristate Acetate Roller Bottle DEAE Column 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Gale A. Granger
    • 1
  • Diane L. Johnson
    • 1
  • J. Michael Plunkett
    • 1
  • Irene K. Masunaka
    • 1
  • Sally L. Orr
    • 1
  • Robert S. Yamamoto
    • 1
  1. 1.Department of Molecular Biology and BiochemistryUniversity of CaliforniaIrvineUSA

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