Isolation, Structural Studies, and Biological Activities
  • Teresa L. K. Low
  • Allan L. Goldstein
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


The studies in the early 1960s by Miller (Miller, 1961), and Good (Good et al., 1962) have established that the thymus is necessary for the normal development of the immune response. For the past 20 years, a large research effort has been directed toward the isolation and identification of the thymic factors or hormones responsible for the physiological functions of the thymus gland (Low and Goldstein, 1978). Our previous studies demonstrated that a partially purified bovine thymic preparation termed thymosin fraction 5 could partially or fully reconstitute immune functions in immunodeficient diseases, autoimmune diseases, and cancer.


Mixed Lymphocyte Reaction Minimal Change Nephrotic Syndrome Complete Amino Acid Sequence Thymic Hormone Polypeptide Component 
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  1. Abiko, T., Onodera, I., and Sekino, H., 1980, Synthesis and immunological effects of thymosin α1 and its fragments on inhibitory factor in minimal changes nephrotic syndrome, Chem. Pharm. Bull. 28:3542.PubMedCrossRefGoogle Scholar
  2. Bach, J.-F., Dardenne, M., and Pléau, J. M., 1977, Biochemical characterization of a serum thymic factor, Nature (London) 266:55.CrossRefGoogle Scholar
  3. Birr, C., and Stollenwerk, U., 1979, Synthesis of thymosin α1, a polypeptide of the thymus, Angew. Chem. Int. Ed. Engl. 18:394.PubMedCrossRefGoogle Scholar
  4. Bistoni, F., Marconi, P., Frati, L., Bonmassar, E., and Garaci, E., 1982, Increase of mouse resistance to Candida albicans infection by thymosin α1, Infect. Immun. 36:609.PubMedGoogle Scholar
  5. Chou, P. Y., and Fasman, G. D., 1974, Conformational parameters for amino acids in helical, β-sheet and random coil regions calculated from proteins, Biochemistry 13:211.PubMedCrossRefGoogle Scholar
  6. Ciardelli, T. L., Incefy, G. S., and Birr, C., 1982, Activity of synthetic thymosin α1 C-terminal peptides in the azathioprine E-rosette inhibition assay, Biochemistry 21:4233.PubMedCrossRefGoogle Scholar
  7. Colombo, R., 1981, Solid-phase peptide synthesis without acidolysis: The synthesis of thymosin α1 on a new benzhydrylamine resin, J. Chem. Soc. Chem. Commun. 1981:1012.CrossRefGoogle Scholar
  8. Folkers, K., Leban, J., Sakura, N., Rampold, G., Lundanes, E., Dahmen, J., Lebek, M., Ohta, J., and Bowers, C. Y., 1980, Current advances on biologically active synthetic peptides, in: Polypeptide Hormones (R. F. Beers and E. G. Bassett, eds.), p. 149, Raven Press, New York.Google Scholar
  9. Frasca, D., Garavini, M., and Doria, G., 1982, Recovery of T-cell functions in aged mice injected with synthetic thymosin α1, Cell. Immunol. 72:384.PubMedCrossRefGoogle Scholar
  10. Freire, M., Hannappel, E., Rey, M., Freire, J. M, Kido, H., and Horecker, B. L., 1981, Purification of thymus mRNA coding for a 16,000-dalton polypeptide containing the thymosin α1 sequence, Proc. Natl. Acad. Sci. USA 78:192.PubMedCrossRefGoogle Scholar
  11. Good, R. A., Dalmasso, A. P., Martinez, G., Archer, O. K., Pierce, J. C., and Papermaster, B. W., 1962, The role of the thymus in development of immunologic capacity in rabbits and mice, J. Exp. Med. 116:773.PubMedCrossRefGoogle Scholar
  12. Hannappel, E., Davoust, S., and Horecker, B. L., 1982, Thymosins β8 and β9: Two new peptides isolated from calf thymus homologous to thymosin β4, Proc. Natl. Acad. Sci. USA 79:1708.PubMedCrossRefGoogle Scholar
  13. Hu, S. K., 1980, Studies on thymosin: (1) Regulation of terminal deoxynucleotidyl transferase activity; (2) Evaluation of chemical and biological relationships among species, Ph.D. dissertation, The University of Texas Medical Branch, Galveston.Google Scholar
  14. Hu, S. K., Low, T. L. K., and Goldstein, A. L., 1981, Modulation of terminal deoxynucleotidyl transferase activity by thymosin, Mol. Cell. Biochem. 41:49.PubMedCrossRefGoogle Scholar
  15. Kido, H., Vita, A., Hannappel, E., and Horecker, B. L., 1981, Aminoterminal acetylation of synthetic iVa-desacetyl thymosin α1, Arch. Biochem. Biophys. 208:101.CrossRefGoogle Scholar
  16. Kook, A. I., Yakir, Y., and Trainin, N., 1975, Isolation and partial chemical characterization of THF, a thymus hormone involved in immune maturation of lymphoid cells, Cell. Immunol. 19:151.PubMedCrossRefGoogle Scholar
  17. Low, T. L. K., and Goldstein, A. L., 1978, Structure and function of thymosin and other thymic factors, in: The Year in Hematology (R. Silber, J. Lobue, and A. S. Gordon, eds.), pp. 281–319, Plenum Press, New York.Google Scholar
  18. Low, T. L. K., and Goldstein, A. L., 1979, The chemistry and biology of thymosin. II. Amino acid sequence analysis of thymosin α1 and polypeptide β1, J. Biol. Chem. 254:987.PubMedGoogle Scholar
  19. Low, T. L. K., and Goldstein, A. L., 1982, Chemical characterization of thymosin β4, J. Biol. Chem. 257:1000.PubMedGoogle Scholar
  20. Low, T. L. K., Thurman, G. B., McAdoo, M., McClure, J., Rossio, J., Naylor, P. H., and Goldstein, A. L., 1979, The chemistry and biology of thymosin. I. Isolation, characterization and biological activities of thymosin α1 and polypeptide β1 from calf thymus, J. Biol. Chem. 254:981.PubMedGoogle Scholar
  21. Low, T. L. K., McClure, J. E., Naylor, P. H., Spangelo, B. L., and Goldstein, A. L., 1983a, Isolation of thymosin α1 from thymosin fraction 5 of different species by high performance liquid chromatography, J. Chromatogr. 266:533.PubMedCrossRefGoogle Scholar
  22. Low, T. L. K., Seals, C., and Goldstein, A. L., 1983b, Isolation of a thymic polypeptide active in mixed lymphocyte reaction (MLR), Fed. Proc. 42:1873.Google Scholar
  23. Low, T. L. K., Wang, S. S., and Goldstein, A. L., 1983c, Solid-phase synthesis of thymosin β4: Chemical and biological characterization of the synthetic peptide, Biochemistry 22:733.PubMedCrossRefGoogle Scholar
  24. McClure, J. E., Lameris, N., Wara, D. W., and Goldstein, A. L., 1982, Immunochemical studies on thymosin: Radioimmunoassay of thymosin α1, J. Immunol. 128:368.PubMedGoogle Scholar
  25. Miller, J. F. A. P., 1961, Immunological function of the thymus, Lancet 2:748.PubMedCrossRefGoogle Scholar
  26. Neta, R., and Salvin, S. B., 1983, Resistance and susceptibility to infection in inbred murine strains, Cell. Immunol. 75:173.PubMedCrossRefGoogle Scholar
  27. Schlesinger, D. H., and Goldstein, G., 1975, The amino acid sequence of thymopoietin II, Cell 5:361.PubMedCrossRefGoogle Scholar
  28. Schlesinger, D. H., Goldstein, G., and Niall, H. D., 1975, The complete amino acid sequence of ubiquitin, and adenylate cyclase stimulating polypeptide probably universal in living cells, Biochemistry 14:2214.PubMedCrossRefGoogle Scholar
  29. Thurman, G. B., Low, T. L. K., Rossio, J. L., and Goldstein, A. L., 1981, Specific and non-specific macrophage migration inhibition, in: Lymphokines and Thymic Hormones: Their Potential in Cancer Therapeutics (A. L. Goldstein and M. Chirigos, eds.), p. 145, Raven Press, New York.Google Scholar
  30. Wang, S. S., Kulesha, I. D., and Winter, D. P., 1978, Synthesis of thymosin α1, J. Am. Chem. Soc. 101:253.CrossRefGoogle Scholar
  31. Wang, S. S., Makosfke, R., Bach, A., and Merrifield, R. B., 1981, Automated solid phase synthesis of thymosin α1, Int. J. Pept. Protein Res. 15:1.CrossRefGoogle Scholar
  32. Wetzel, R., Heyneker, H. L., Goeddel, D. V., Jhurani, P., Shapiro, J., Crea, R., Low, T. L. K., McClure, J. E., and Goldstein, A. L., 1980, Production of biologically active Nα-desacetylthymosin α1 in E. coli through expression of a chemically synthesized gene, Biochemistry 19:6096.PubMedCrossRefGoogle Scholar
  33. Wilkinson, K. D., Urban, M. K., and Haas, A. L., 1980, Ubiquitin is the ATP-dependent proteolysis factor 1 of rabbit reticulocytes, J. Biol. Chem. 255:7529.PubMedGoogle Scholar
  34. Wong, T. W., and Merrifield, R. B., 1980, Solid-phase synthesis of thymosin α1 using tert-butyloxycarbonylaminoacyl-4(oxymethyl)phenylacetamidomethyl-resin, Biochemistry 19:3233.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Teresa L. K. Low
    • 1
  • Allan L. Goldstein
    • 1
  1. 1.Department of BiochemistryThe George Washington University School of Medicine and Health SciencesUSA

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