Advertisement

The Synthesis and Physical Properties of Thymosin α1 and Its Analogs Prepared by Fragment Condensation

  • Arthur M. Felix
  • Edgar P. Heimer
  • Ching-Tso Wang
  • Theodore J. Lambros
  • Joseph Swistok
  • Mushtaq Ahmad
  • Martin Roszkowski
  • Arnold Trzeciak
  • Dieter Gillessen
  • Voldemar Toome
  • Bogda Wegrzynski
  • Ross Pitcher
  • Johannes Meienhofer
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

The isolation of biologically important peptides from the thymus gland has been studied extensively in the last few years. Several thymic peptides have been shown to play certain roles in T-cell maturation (White, 1980; Trainin et al., 1980a, b; Goldstein and Lau, 1980; Bach and Goldstein, 1980). Thymosin α1, a highly acidic Nα-acetyl octacosapeptide, isolated from calf thymus gland (Goldstein et al., 1977) and characterized by sequence analysis (Low and Goldstein, 1979), has been reported to exhibit biological activities involved in the development of thymus-dependent lymphocytes (T cells) (Goldstein et al., 1977). Thymosin a1 has been synthesized by classical procedures in solution (Wang et al., 1979; Birr and Stollenwerk, 1979) and by solid-phase methods (Wong and Merrifield, 1980; Wang et al., 1980; Colombo, 1981).

Keywords

Circular Dichroism Solid Phase Synthesis Preparative HPLC Thymus Gland Thymic Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bach, J.-F., and Goldstein, G., 1980, Newer concepts of thymic hormones, Thymus 2:1–4.Google Scholar
  2. Birr, C., and Stollenwerk, U., 1979, Synthesis of thymosin on, a polypeptide of the thymus, Angew. Chem. Int. Ed. Engl. 18:394–395.PubMedCrossRefGoogle Scholar
  3. Colombo, R., 1981, Solid-phase peptide synthesis without acidolysis: The synthesis of thymosin α1 on a new benzhydrylamine resin, J. Chem. Soc. Chem. Commun. 1981:1012–1013.CrossRefGoogle Scholar
  4. Goldstein, A. L., Low, T. L. K., McAdoo, M., McClure, J. E., Thurman, G. B., Rossio, J. L., Lai, C.-Y., Chang, D., Wang, S.-S., Harvey, C., Ramel, A. H., and Meienhofer, J., 1977, Thymosin α1 Isolation and sequence analysis of an immunologically active thymic polypeptide, Proc. Natl. Acad. Sci. USA 74:725–729.PubMedCrossRefGoogle Scholar
  5. Goldstein, G., and Lau, C., 1980, Thymopoietin and immunoregulation, in: Polypeptide Hormones (R.F. Beers, Jr., and E.G. Bassett, eds.), pp. 459–465, Raven Press, New York.Google Scholar
  6. Ishitsuka, H., Umeda, Y., Nakamura, J., and Yagi, 1983, Protective activity of thymosin against opportunistic infections in animal models, Cancer Immunol. Immunother. 14:145–150.PubMedCrossRefGoogle Scholar
  7. Low, T. L. K., and Goldstein, A. L., 1979, The chemistry and biology of thymosin, J. Biol. Chem. 254:987–993.PubMedGoogle Scholar
  8. Trainin, N., Umiel, T., Klein, B., and Kleir, I., 1980a, Role of thymus humoral factor, a thymic hormone, in the physiology of the thymus, in: Polypeptide Hormones (R.F. Beers, Jr., and E.G. Bassett, eds.), pp. 467–488, Raven Press, New York.Google Scholar
  9. Trainin, N., Zaisov, R., Yakir, Y., and Rotter, V., 1980b, Thymic hormones: Characterization and perspectives, in: The Immune System: Functions and Therapy of Dysfunction (G. Doria and A. Eshkol, eds.), pp. 159–169, Academic Press, New York.Google Scholar
  10. Wang, S.-S., Kulesha, I. D., and Winter, D. P., 1979, Synthesis of thymosin on, J. Am. Chem. Soc. 101:253–254.CrossRefGoogle Scholar
  11. Wang, S.-S., Makofske, R., Bach, A., and Merrifield, R. B., 1980, Automated solid phase synthesis of thymosin ai, Int. J. Pept. Protein Res. 15:1–4.PubMedCrossRefGoogle Scholar
  12. White, A., 1980, Chemistry and biological actions of products with thymic hormone-like activity, in: Biochemical Actions of Hormones, Vol. VII, (G. Litwack, ed.) pp. 1–46, Academic Press, New York.Google Scholar
  13. Wong, T. W., and Merrifield, R. B., 1980, Solid phase synthesis of thymosin a1 using tert-butyloxy-carbonyl-aminoacyl-4-(oxymethyl)-phenylacetamidomethyl-resin, Biochemistry 19:3233–3238.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Arthur M. Felix
    • 1
  • Edgar P. Heimer
    • 1
  • Ching-Tso Wang
    • 1
  • Theodore J. Lambros
    • 1
  • Joseph Swistok
    • 1
  • Mushtaq Ahmad
    • 1
  • Martin Roszkowski
    • 1
  • Arnold Trzeciak
    • 2
  • Dieter Gillessen
    • 2
  • Voldemar Toome
    • 1
  • Bogda Wegrzynski
    • 1
  • Ross Pitcher
    • 1
  • Johannes Meienhofer
    • 1
  1. 1.Bio-Organic Chemistry Department, Roche Research CenterHoffman-La Roche Inc.NutleyUSA
  2. 2.F.Hoffmann-La Roche and Co., Ltd.BaselSwitzerland

Personalised recommendations