Suppressor T-Cell Hybridoma with a Receptor Recognizing KLH-Specific Suppressor Factor
Immune responses are known to be regulated by complicated interactions of T-cell subsets that play different functional roles.(1–6) In our previous studies of the cellular mechanisms in the suppression of antibody response, at least three functionally distinct subsets of suppressor T cells, i.e., suppressor T cells producing antigen-specific factor (TsF), suppressor T cells with acceptor site for TsF (acceptor T cell), and effector-suppressor T cells, were found to be involved in the regulatory T-cell interactions.(1) The initial step of suppressor T cell interaction is a process of activation of acceptor T cells by an antigen-specific TsF. The effector-suppressor T cells are then generated under the influence of activated acceptor T cells. Therefore, antigen-specific TsF mediates the regulatory T-cell interaction in which no direct cell contact is necessary. Moreover, antigen-specific TsF seems not to be an effector-suppressor molecule but rather a molecule whose function is to activate other subsets of suppressor T cells. In this sense, the antigen-specific TsF appears to work as a device to recognize antigens and also to communicate with other T cells.
KeywordsCellulose Sucrose Electrophoresis Polypeptide Polyacrylamide
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- 2.Waltenbaugh, C., Theze, J., Kapp, J. A., and Benacerraf, B., 1977, Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyrosine50 (GT). III. Generation of suppressor T cells by a suppressive extract derived from GT-primed lymphoid cells, J. Exp. Med. 146:970–985.PubMedCrossRefGoogle Scholar
- 5.Germain, R. N., Theze, J., Waltenbaugh, C., Dorf, M. E., and Benacerraf, B., 1978, Antigen specific T cell mediated suppression. II. In vitro induction of I-J coded L-glutamic acid50-L-tyrosine50 (GT)-specific T cell suppressor factor (GT-TsF) of suppressor T cells (Ts2) bearing distinct I-J determinants, J. Immunol. 121:602–607.PubMedGoogle Scholar
- 6.Sy, M.-S., Dietz, M. H., Germain, R. N., Benacerraf, B., and Greene, M. I., 1980, Antigen- and receptor- driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induced second-order suppressor T cells which express anti-idiotypic receptors,J. Exp. Med. 151:1183–1195.PubMedCrossRefGoogle Scholar
- 9.Saito, T., and Taniguchi, M., 1983, Chemical features of an antigen-specific suppressor T cell factor composed of two polypeptide chains, submitted J. Mol. Cell. Immunol. (in press).Google Scholar
- 15.Taniguchi, M., Takei, I., Saito, T., and Tokuhisa, T., 1981, Activation of an acceptor T cell hybridoma by a VH I-J+ monoclonal suppressor factor, in: Immunoglobulin idiotypes (C. A. Janeway, Jr., E. E. Sercarz, and H. Wigzell, eds.), Academic Press, New York, pp. 397–406.Google Scholar