Quantitative Estimates of Diversity, Degeneracy, and Connectivity in an Idiotypic Network among T Cells
We have previously described a series of limiting dilution experiments in which several T-cell types of a number of different antigenic specificties were analyzed(1–5) In these studies, we obtained data on the precursor frequencies of antigen-recognizing T-helper cells, antigen-recognizing cytotoxic T cells, and of suppressor T cells that regulate their differentiation and expression as functional effector cells (reviewed in Ref. 5). Thus, these experiments not only provided information on T-cell precursor frequencies in normal and immunized mice but also on their regulation by suppression.(5,6) The quantitative and qualitative data obtained in these experiments strongly suggest that a suppressive network exists among normal T cells, and the data can be used to generate estimates on the diversity and degeneracy of receptors as well as on the connectivity in such a network.
KeywordsResponse Mode Suppressor Cell Canonical Partition Function Virgin State Precursor Frequency
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- 1.Eichmann, K., Falk, I., Melchers, I., and Simon, M. M., 1980, Quantitative studies on T cell diversity. I. Determination of the precursor frequencies of two types of streptococcus A-specific helper cells in nonimmune, polyclonally activated splenic T cells, J. Exp. Med. 152:477.PubMedCrossRefGoogle Scholar
- 2.Goronzy, J., Schafer, U., Eichmann, K., and Simon, M. M., 1981, Quantitative studies on T cell diversity. II. Determination of the frequencies and Lyt phenotypes of two types of precursor cells for alloreactive cytotoxic T cells in polyclonally and specifically activated splenic T cells, J. Exp. Med. 153:857.PubMedCrossRefGoogle Scholar
- 4.Eichmann, K., Goronzy, J., Hamann, U., Krammer, P. H., Kuppers, R., Melchers, I., Simon, M. M., and Zahn, G., 1982, Clonal analysis of helper and cytolytic T cells: Multiple independently regulated precursor sets at frequencies suggesting a limited repertoire, in:Isolation, Characterization and Utilization of T Lymphocyte Clones (C. G. Fathman and F. W. Fitch, eds.), Academic Press, New York, p. 233.Google Scholar
- 8.Weyand, C., Goronzy, J., and Hämmerling, G., 1981, Recognition of polymorphic H-2 domains by T lymphocytes. I. Functional role of different H-2 domains for the generation of alloreactive cytotoxic T lymphocytes and determination of precursor frequencies, J. Exp. Med. 154:1717.PubMedCrossRefGoogle Scholar
- 10.Hoffmann, G., 1982, The application of stability criteria in evaluating network regulation models, in:Oscillatory Dynamics in the Immune Response (C. DeLisi and J. Hiernaux, eds.), CRC Press, Boca Raton, Fla., pp. 137–162.Google Scholar
- 11.Landau, L. D., and Lifschitz, E. M., 1966, Theoretische Pysik V, Akademie Verlag, Berlin.Google Scholar
- 12.Huang, K., 1966, Statistical Mechanics, Wiley, New York.Google Scholar
- 13.Becker, R., 1966, Theorie der Wärme, Springer-Verlag, Berlin.Google Scholar