Restriction Endonucleases, DNA Sequencing, and Computers

  • Richard J. Roberts


Among the 250 Type II restriction endonucleases now characterized, there are more than 70 different specificities1 and yet there is no indication that the range of specificities is exausted. Indeed, there is good reason to believe that hundreds, if not thousands, of different specificities would be found if a diligent search were carried out. One reason for this speculation is illustrated in Table 1, which shows the range of sequence patterns with which different Type II restriction endonucleases interact. Among the simple symmetric hexanucleotide sequences designated here as Class A, almost half of the possible sequence patterns are already represented by well-characterized enzymes. There is no reason to believe that a similar number of enzymes will not be found for the other patterns in Classes B through F. Similarly, it seems likely that enzymes recognizing degenerate patterns, like HgiAI and AccI, are not the sole representatives of the class. Within the last year alone, five new classes (C, D, F, N, and O) were added to this list.


Sequence Pattern Sequence File Diligent Search Clone Bank Dyad Axis 


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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Richard J. Roberts
    • 1
  1. 1.Cold Spring Harbor LaboratoryCold Spring HarborUSA

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