Photosensitizing Properties of Free and Bound Uroporphyrin I

  • John D. Spikes
  • Bruce F. Burnham
  • Jerry C. Bommer


Most studies of the sensitized photooxidation of biomolecules have been carried out with the sensitizer in solution or, as in the case of many porphyrins, in the form of small aggregates suspended in the medium.1 Cells, however are not homogeneous; they consist of a wide variety of microenvironments with different physical-chemical properties. Thus most photosensitizers added to cellular systems, depending on their properties, would be expected to dissolve in particular microregions of the cells or bind to cellular macromolecules such as proteins, nucleic acids or carbohydrates.2 This could change the sensitizer properties, the efficiency of photosensitization and the sensitizing mechanism.2,3 Thus it is difficult to carry over information on photosensitizers as obtained in solution to the cellular-organismal level. One preliminary approach to this problem is to examine the photochemical behavior of photosensitizer molecules bound to surfaces to simulate the possible intracellular situation. The present paper describes a preliminary comparison of the photosensitizing properties of uroporphyrin I in aqueous solution and bound covalently to beads of an agarose gel.


Quantum Yield Rose Bengal Furfuryl Alcohol Photosensitize Oxidation Photosensitize Property 


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  1. 1.
    E. Reddi, E. Rossi, and G. Jori, Factors controlling the efficiency of porphyrins as photosensitizers of biological systems to damage by visible light, Med. Biol. Environ. 9:337 (1981).Google Scholar
  2. 2.
    G. Jori and J. D. Spikes, Photosensitized oxidations in complex biological structures, pp. 441–457 in: “Oxygen and Oxy-Radicals in Chemistry and Biology”, M. A. J. Rodgers and E. L. Powers, eds., Academic Press, New York (1981).Google Scholar
  3. 3.
    R. C. Straight, Photophysical, photochemical and photodynamic properties of bound sensitizers, Program Amer. Soc. Photobiol. 10:90 (1982) (and references therein).Google Scholar
  4. 4.
    P. Cuetrecasas and C. B. Anfinsen, Affinity chromatography, pp. 345–378 in: “Methods in Enzymology”, Vol. 22, W. B. Jacoby, ed., Academic Press, New York (1971).Google Scholar
  5. 5.
    F. Rizzuto and J. D. Spikes, The eosin-sensitized photo-oxidation of substituted phenylalanines and tyrosines, Photochem. Photobiol. 25:465 (1977).CrossRefGoogle Scholar
  6. 6.
    J. S. Bellin, Photophysical and photochemical effects of dye binding, Photochem. Photobiol. 8:383 (1968).CrossRefGoogle Scholar
  7. 7.
    G. Jori and J. D. Spikes, The photobiochemistry of porphyrins, in: “Topics in Photomedicine”, K. C. Smith, ed., Plenum Publishing Corp., New York, in press.Google Scholar
  8. 8.
    A. P. Schapp, A. L. Thayer, E. C. Blossey, and D. C. Neckers, Polymer-based sensitizers for photooxidation. II, J. Am. Chem. Soc. 97:3741 (1975).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • John D. Spikes
    • 1
  • Bruce F. Burnham
    • 2
  • Jerry C. Bommer
    • 2
  1. 1.Department of BiologyUniversity of UtahSalt Lake CityUSA
  2. 2.Porphyrin Products, Inc.LoganUSA

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