On the Purification of Hematoporphyrin IX and its Acetylated Derivatives
Hematoporphyrin IX (Hp, Fig. 1a) and especially the related product known as “Hematoporphyrin Derivative or HpD” have been the most used compounds in tumor diagnostic and phototherapy as outlined during this symposium. Also, hematoporphyrin is one of the most common photosensitizer. This situation is disquieting because of the general uncertainty about the purity of hematoporphyrin1 The major impurities of the commercial product are 8(3)-(1-hydro-xyethyl)-3(8)-vinyldeuteroporphyrin (HVD, 2 isomers, Fig. 1b), protoporphyrin (PP, Fig. 1c) and unidentified brown impurities.
KeywordsGeneral Uncertainty Chloroformic Phase Major Impurity Eluent Flow Rate Hematoporphyrin Derivative
Unable to display preview. Download preview PDF.
- 1.R. K. Di Nello and C.K. Chang, Thuong in: “The Porphyrin” Vol. 1, Academic Press, New York (1978),Google Scholar
- 2.T.J. Dougherty, G. Lawrence, J.H. Kaufman, D. Boyle, K.R. Weishaupt, and A. Goldfarb, Photoradiation in the treatment of recurrent breast carcinoma, J. Natl. Cancer Inst. 62:231 (1979).Google Scholar
- 4.R. Bonnett, R.J. Ridge, P.A. Scourides, and M.C. Berenbaum, On the nature of Hematoporphyrin Derivative, J. Chem. Soc. Perkin I. 3135 (1981).Google Scholar
- 7.T.J. Dougherty, D.G. Boyle, K.R. Weishaupt, B.A. Henderson, W.R. Potter, D.A. Bellnier, and K.E. Wityk, Photoradiation therapy; clinical and drug advances, in: “Porphyrin Photo-sensitization”, D. Kessel and T.J. Dougherty, ed., Plenum Press, New York-London (1983).Google Scholar
- 8.T.J. Dougherty, Variability in Hematoporphyrin Derivative preparation, Cancer Res. 42:1188 (1982).Google Scholar
- 9.M. Momenteau, C. Ropars, and M. Rougee, J. Chim. Phys. Chim. Biol. 65:1635 (1968).Google Scholar