Photodynamic Effect of the He-Ne Laser with HpD on the Ultrastructure of Rhabdomyosarcoma Cell
Photodynamic effect of Hematoporphyrin Derivative (HpD) on malignant cells has been widely studied; cells which have taken up HpD are killed “in vitro” or “in vivo”, when the compound is activated by light. The light excitation of HpD produces singlet oxygen which, by its strong oxidizing power, breaks down the structure and alters the biological function of major biomolecules, including proteins, nucleic acids, cholesterol, fibrinogen, and various kind of enzymes etc. 1,2. As all of these are the essential materials in the structure and function of the cell, photodynamic action of this kind may induce extensive damage or alteration in the ultra-structure of cells. It has been reported that photodynamic action dissolves the cell membrane, oxidizes phospholipid and cholesterol, causes damage of enzymes in membrane transferring system, inhibits Ca++ transportation of Chinese hamster ovarian cells 3, depresses oxidative phosphorylation in mitochondria as well as oxygen consumption 4, breaks down on DNA chain 5, and kills the tumor cells 6 especially those at G1-S stage of the cell cycle 7. The singlet oxygen action causes wide-spread damage of the cell which can be revealed easily by observing the ultrastructure under electron microscopy.
KeywordsAutophagosome Formation Rhabdomyosarcoma Cell Photodynamic Action Secondary Lysosome Photodynamic Effect
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