Cholesterol Impregnation into Erythroleukemia Cell Membrane Induces Resistance to Hematoporphyrin Photodynamic Effect
Specific damage to the membrane of Friend erythroleukemic cells was the result of the hematoporphyrin derivative (HPD) photodynamic effect as analysed by scanning electron microscopy (SEM). Appearance of limited area of tiny holes in the outer membrane was followed by vesicular formation and complete decomposition of the membrane, while bare nucleus remained undamaged. In addition, a decrease in cell number as a function of HPD concentration used, as well as a reduction in protein synthesis, were detected in HPD-treated Friend cells. Furthermore, a parallel inhibition of virus release from these cells was also observed, as determined by the reverse transcriptase assay. Preincubation of the erythroleukemic cells with cholesterol hemisuccinate induced almost complete resistance to the HPD photodynamic damage and protein synthesis rate remained similar to control unsensitized cells.
KeywordsProtein Synthesis Rate Reverse Transcriptase Activity Virus Release Photodynamic Effect Cholesterol Impregnation
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