Photodynamic Inactivation of L929 Cells after Treatment with Hematoporphyrin Derivative
Porphyrin photoradiation therapy for the treatment of various malignant tumors is a recent rapidly developing technique and progress has been fast and promising1,2). The principle of porphyrin photoradiation therapy is relatively simple. Following systematic administration, porphyrins, especially the so-called hematoporphyrin-derivative (HpD) are accumulated to higher concentrations in malignant than in normal cells3). During subsequent exposure of the cells to visible light the accumulated porphyrin acts as photodynamic sensitizer 4) and causes oxidation of cellular constituent presumably by formation of singlet oxygen and possibly hydroxyl radicals. These oxidations lead to disturbed functions and, ultimately, cell death. Three mechanisms may be responsible e.g. membrane deterioration, DNA-damage and photodynamic inactivation of crucial enzyme systems. Studies of Kessel et al. indicate that membrane damage may be the direct cause of photodynamic cell death in tumors 5–7). Illumination of L1210 and SS-1 cells incubated with porphyrin leads to cross-linking of membrane proteins 6) as described before with red cell membranes 8,9). Further,loss of cell viability was correlated with inhibition of transmembrane cycloleucine transport 5).
KeywordsL929 Cell Illumination Time Hexokinase Activity Photodynamic Action Photodynamic Effect
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