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Fluorescence of Porphyrins in Cells

  • Johan Moan

Abstract

Two fields of research in biology and medicine are concerned with the study of cellular uptake of porphyrins: research on porphyria and research on diagnosis and photoradiation therapy of cancer. Porphyrins have a characteristic pattern of fluorescence. Thus, cellular uptake and retention of porphyrins are often studied by means of fluorescence measurements.

Keywords

Cellular Uptake Fluorescence Quantum Yield Fluorescence Emission Spectrum Fluorescence Excitation Spectrum Increase Incubation Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    D. Kessel and K.I. Kohn, Transport and binding of mesoporphyrin IX by leukemia L 1210 cells, Cancer Res. 40:303 (1980).Google Scholar
  2. 2.
    D. Kessel and E. Rossi, Determinants of porphyrin-sensitized photooxidation characterized by fluorescence and absorption spectra, Photochem. Photobiol. 35:37 (1982).CrossRefGoogle Scholar
  3. 3.
    A.A. Lamola, I. Asher, U. Muller-Eberhard and M. PoleFitzpatrick, Fluorometric study of the binding of photopor-phyrin to haemopexin and albumin. Biochem. J. 196:693 (1981).Google Scholar
  4. 4.
    W.T. Morgan, A. Smith and P. Koskelo, The interaction of human serum albumin and hemopexin with porphyrins. Biochem. Biophys. Acta 624:271 (1980).Google Scholar
  5. 5.
    R.L. Lipson, E.J. Baldes and A.M. Olsen, The use of a derivative of hematoporphyrin in tumor detection. J. Natl. Cancer Inst. 26:1 (1961).Google Scholar
  6. 6.
    J. Moan, E.O. Pettersen and T. Christensen, The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin. Br. J. Cancer 39:398 (1979).CrossRefGoogle Scholar
  7. 7.
    J. Moan, S. Sandberg, T. Christensen and S. Elander, Hematoporphyrin derivative: Chemical composition and photosensitizing proporties, in: “Porphyrin Photosensitization”, D. Kessel and T.J. Dougherty, Plenum Press, New York, London (1983).Google Scholar
  8. 8.
    R. Bonnett and R. Berenbaum, HPD — a study of its components and their properties, in: “Porphyrin Photosensitization”, D. Kessel and T.F. Dougherty, Plenum Press, New York and London (1983).Google Scholar
  9. 9.
    T.J. Dougherty, D.G. Boyle, K.R. Weishaupt, B.A. Henderson, W.R. Potter, D.A. Bellnier and K.E. Wityk, Photoradiation therapy — clinical and drug advances, in: “Porphyrin Photo-sensitization”, Plenum Press, New York and London (1983)Google Scholar
  10. 10.
    A. Andreoni, R. Cubeddu, S. de Silvestri, P. Laport, G. Jori and E. Reddi, Hematoporphyrin derivative: Experimental evidence for aggregated species. Chem. Phys. Lett. 88:33 (1982).CrossRefGoogle Scholar
  11. 11.
    S.B. Brown, M. Shillcock and P. Jones, Equilibrium and kinetic studies of the aggregation of porphyrins in aqueous solutions. Biochem. J. 153:279 (1976).Google Scholar
  12. 12.
    J. Moan, J.B. McGhie and T. Christensen, Hematoporphyrin derivative: Photosensitizing efficiency and cellular uptake of its components. Photobiochem. Photobiophys. 4:337 (1982).Google Scholar
  13. 13.
    D. Kessel, Effects of photoactivated porphyrins at the cell surface of leukemia L 1210 cells. Biochem. 16:3443 (1977).CrossRefGoogle Scholar
  14. 14.
    B.W. Henderson, D.A. Bellnier, B. Ziring and T.J. Dougherty, Aspects of the cellular uptake and retention of hematoporphyrin derivative and their correlation with the biologic responses to PRT in vitro, in: “Porphyrin Photosensitiza-tion”, D. Kessel and T.J. Dougherty, Plenum Press, New York and London (1983).Google Scholar
  15. 15.
    J. Moan, J.V. Johannessen, T. Christensen, T. Espevik and J.B. McGhie, Porphyrin-sensitized photoinactiyation of human cells in vitro, Am. J. Pathol. 109:184 (1982).Google Scholar
  16. 16.
    T. Christensen, T. Sandquist, K. Feren, H. Waksvik and J. Moan, Retention and photodynamic effects of haematoporphyrin derivative in cells after prolonged cultivation in the presence of porphyrin. Br. J. Cancer, in press.Google Scholar
  17. 17.
    J. Moan and T. Christensen, Cellular uptake and photodynamic effect of hematoporphyrin. Photobiochem. Photobiophys. 2:291 (1981).Google Scholar
  18. 18.
    D.R. Doiron, L.O. Svaasand and A.E. Profio, Light dosimetry in tissue: Application to photoradiation therapy, in.: “Porphyrin Photosensitization”, Plenum Press, New York and London (1983).Google Scholar
  19. 19.
    G.S. Cox, C. Bobillier and D. Whitten, Photooxidation and singlet oxygen sensitization by protoporphyrin IX and its photo-oxidation products. Photochem. Photobiol. 36:401 (1982).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Johan Moan
    • 1
  1. 1.Norsk Hydro’s Institute for Cancer ResearchMontebello, Oslo 3Norway

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