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Membrane-Associated Benzoate-4-Hydroxylase from Rhodotorula Graminis

  • Clyde McNamee
  • Don R. Durham
Part of the Basic Life Sciences book series (BLSC, volume 28)

Abstract

Growth of the yeast Rhodotorula graminis on benzoate results in the induction of benzoate hydroxylase, p-hydroxybenzoate-3-hydroxy-lase, protocatechuate-3,4-dioxygenase, and enzymes of the ß-ketoadi-pate pathway. Following subcellular fractionation of crude extracts of benzoate-grown cells, benzoate hydroxylase activity was observed in the particulate (membrane) fraction. Thus, washed membrane preparations, which are devoid of other benzoate-dissimilatory enzymes, were used as a source of partially purified benzoate hydroxylase for further study.

Keywords

Thin Layer Chromatography Environmental Pollutant Crude Extract Genetic Control Enzyme Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Clyde McNamee
    • 1
  • Don R. Durham
    • 1
  1. 1.Genex CorporationGaithersburgUSA

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