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Dissimilation of Aromatic Compounds by Rhodotorula Graminis

  • Clyde McNamee
  • Richard Wattam
  • Jeannette Flickinger
  • David Stewart
  • Don R. Durham
Part of the Basic Life Sciences book series (BLSC, volume 28)

Abstract

The yeast Rhodotorula graminis utilizes benzoate, D,L-mandelate, phenylalanine, and salicylate as sole sources of carbon and energy. Pathways for phenylalanine and mandelate catabolism converge at benzoate; growth of R. graminis on phenylalanine, man-delate, and benzoate elicits the synthesis of benzoate-4-hydroxy-lase, £-hydroxybenzoate-3-hydroxylase, and protocatechuate-3,4-dioxygenase; these enzymes were not detected in crude extracts prepared from salicylate-grown cells. Mutants deficient in either £-hydroxybenzoate-3-hydroxylase or protocatechuate-3,4-dioxygenase were unable to utilize benzoate, phenylalanine, or mandelate; salicylate, however, did support growth. Revertants obtained from either class of mutants resulted in wild-type growth on benzoate, phenylalanine, and mandelate.

Keywords

Environmental Pollutant Crude Extract Aromatic Compound Genetic Control Alternate Pathway 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Clyde McNamee
    • 1
  • Richard Wattam
    • 1
  • Jeannette Flickinger
    • 1
  • David Stewart
    • 1
  • Don R. Durham
    • 1
  1. 1.Genex CorporationGaithersburgUSA

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