Pseudomonas Hydrocarbon Oxidation
In this paper we will withdraw from the complex world of mixed cultures and multiple substrates that characterize polluted ecosystems and treatment facilities to discuss a simple model system involving petri dishes, vapor phase pure substrates, and genetically defined bacterial strains. The laboratory situation is no less “real” than any other and has many important lessons to teach us if we are ever to be in a position to design pollutant-degrading organisms with the same level of sophistication now employed to produce medically important polypeptides. One of the major points to be made is that even a hydrocarbon oxidation system chosen for maximum simplicity reveals a genetic complexity that is beyond our current understanding.
KeywordsAliphatic Alcohol Hydrocarbon Oxidation lacZ Fusion Alkane Hydroxylase Alcohol Dehydrogenase Activity
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