Laboratory Details in an In Vitro Fertilization and Embryo Transfer Program
In this chapter, the details of the IVF-ET program at UT-Houston are presented. This is done not only to introduce the complexities of IVF-ET but also with the objective of moving towards a standardization of protocols. Initially, standardization requires a complete description of the techniques, equipment, and supplies employed. Even with the most minute attention to detail, duplication of results by other laboratories will not necessarily be obtained. The protocols described here are certainly not unique to the UT program and credit as well as appreciation is expressed to Dr. Alex Lopata and co-workers (Lopata et al., 1980). Dr. Lopata visited Houston in the very early phases of our program and was kind enough to examine our protocols and modify them where appropriate. Additionally, references have been added throughout the chapter where modifications can be directly attributed to the contributions of others. IVF-ET is an exceedingly complex undertaking and the parameters which may contribute to success are virtually infinite.
KeywordsSperm Concentration Pasteur Pipette Motile Sperm Laminar Flow Hood Preimplantation Development
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