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The Dependence of Force and Velocity on Calcium and Length in Cardiac Muscle Segments

  • Donald A. Martyn
  • Jeff F. Rondinone
  • Lee L. Huntsman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37)

Abstract

The segment length (SL) dependence of force (F) and light load shortening velocity (VL) was determined for central segments of ferret papillary muscles at different extracellular calcium concentrations. Muscles were maintained at 27°C in a physiological solution which contained in mM: NaC1 140; KC1 5.0; MgSO4 1.0; NaH2PO4 1.0; acetate 20; the pH was 7.4. Calcium concentrations were 1.125, 2.25, 4.5 and 9.0 mM.

Total force-segment length relations were determined from both muscle length isometric (auxotonic) and segment isometric contractions, and were found to be the same for each contraction mode. The peak force generated at a particular segment length was independent of both the amount of shortening during a contraction and the initial SL. Increasing extracellu1er Ca2+ shifted the F-SL relation toward greater force and the SL axis intercept toward shorter SL. Maximum peak twitch tension was achieved in 9.0 mM CaZ+ Calcium variations also changed the shape of the total F-SL relation from linear in high Ca2+, to concave in low Ca2+

In order to estimate the active F-SL relations, corrections were made for passive force by two methods. The first assumed that passive force was related to SL, and yielded F-SL relations which were nearly identical to those found for total force. This similarity included the curvature changes observed in different Ca2+ concentrations, a finding which is consistent with the hypothesis that length dependent activation is the cause of force decline at short SL. The second method assumed passive force to be related to muscle length, an approach which would be appropriate if, for example, a connective tissue sheath on the muscle dominated passive behavior. These F-SL curves displayed a plateau above 90% SLmax and appeared to be vertically shifted versions of each other. Such characteristics are consistent with the possible role of an internal load in causing the decline of force at short SL.

VL-SL relations were obtained from load clamps to I mM, imposed at various times during a segment isometric twitch. The results indicate that 1) VL declines linearly with SL below 90% SLmax and 2) VL-SL relations are shifted to higher velocity and shorter SL axis intercepts by increasing Ca2+ The slopes of the VL-SL relations obtained in different calciums are similar Although an internal load could explain the calcium dependence of VL, it would not explain the similarity of the slopes of the VL-SL relations found in different calciums.

Keywords

Cardiac Muscle Papillary Muscle Force Production Segment Length Extracellular Calcium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Allen, D.G. and Kurihara, S. (1979). Calcium transients at different muscle lengths in rat ventricular muscle. J. Physiol 292: 680.Google Scholar
  2. Brutsaert, D.L. (1978). The force-velocity-length-time interrelation of cardiac muscle. CIBA Founda. Symp. 24: 155.Google Scholar
  3. Brutsaert, D.L., Dellerk, N., Goethals, M.A. and Housmans, P.R. (1978). Relaxation of ventricular cardiac muscle. J. Physiol. 283: 469–480.PubMedGoogle Scholar
  4. Caulfield, J.B. and Bony, T.K. (1978). Collagen network of myocardium. Cire. II. 58: 240.Google Scholar
  5. Donald, T.C., Reeves, D.N.S., Reeves, R.C., Walter, A.A. and Hefner, L.L. (1980). Effect of damaged ends in papillary muscle preparations. Am. J. Physiol. 238: H14 - H23.PubMedGoogle Scholar
  6. Edman, K.A.P. Mechanical deactivation induced by active shortening in isolated muscle fibres of the frog. J. Physiol (London) 246: 255–275.Google Scholar
  7. Edman, K.A.P. The velocity of unloaded shortening and its relation to sarcomere length and isometric force in vertebrate muscle fibers. J. Physiol. 291: 143–159.Google Scholar
  8. Fabiato, A. and Fabiato, F. (1975). Dependence of contractile activation of skinned cardiac cells on the sarcomere length. Nature 256: 54–56.PubMedCrossRefGoogle Scholar
  9. Fabiato, A. and Fabiato, F. (1978). Myofilament tension oscillations during partial calcium activation and activation dependence of the sarcomere length tension relation of skinned cardiac cells. J. Gen. Physiol. 72: 867–699.CrossRefGoogle Scholar
  10. Gordon, A.M., Huxley, A.F. and Julian, F.S. (1966). Tension development in highly stretched vertebrate muscle fibres. J. Physiol. London 184: 143–169.Google Scholar
  11. Gordon, A.M. and Ridgeway, E.B. (1978). Calcium transients and relaxation in single muscle fibres. Eur. J. Cardiol. Suppl. 7: 27–34.PubMedGoogle Scholar
  12. Gordon, A.M. and Pollack, G.H. (1980). Effects of calcium on sarcomere length-tension relation in rat cardiac muscle: implications for the Starling-Frank mechanism. Circ. Res. 47 (4): 810–619.CrossRefGoogle Scholar
  13. Hibberd, M.G. and Jewell, B.R. (1979). Length dependence of the sensitivity of the contractile system to calcium in rat ventricular muscle. J. Physiol. 290: 30–31.Google Scholar
  14. Huntsman, L.L., Day, S.R. and Stewart, D.K. (1977). Nonuniform contraction in the isolated eat papillary muscle. Am. J. Physiol. 233 (5): H613 - H618.PubMedGoogle Scholar
  15. Huntsman, L.L., Joseph, D.S., Oiye, M.Y. and Nichols, G.L. (1979). Auxotonic contractions in cardiac muscle segments. Am. J. Physiol. 237: H131 - H139.PubMedGoogle Scholar
  16. Huntsman, L.L. and Stewart, D.K. (1977). Length-dependent calcium inotropism in cat papillary muscle. Cire. Res. 40 (4): 336–371.CrossRefGoogle Scholar
  17. Jewell, B.R. (1977). A reexamination of the influence of muscle length on myocardian performance. Circ. Res. 40: 221–230.CrossRefGoogle Scholar
  18. Julian, F.J. (1971). The effect of calcium on the force-velocity relation of briefly glycerinated frog muscle fibers. J. Physiol. 218: 117–145.PubMedGoogle Scholar
  19. Julian, F.J. and Sollins, M.R. (1975). Sarcomere-length tension relations in living rat papillary muscle. Cire. Res. 37: 299–308.CrossRefGoogle Scholar
  20. Julian, F.S. and Moss, R.L. (1976). Absence of a plateau in length-tension relationship of rabbit papillary muscle when internal shortening is prevented. Nature 260: 340–342.PubMedCrossRefGoogle Scholar
  21. Krueger, J.W. and Pollack, G.H. (1975). Myocardial sarcomere dynamics during isometric contractions. J. Physiol. 25: 627–643.Google Scholar
  22. Lakatta, E.G. and Jewell, B.R. (1977). Length-dependent activation: Its effect on the length-tension relation in cat ventricular muscle. Circ. Res. 40: 251–257.PubMedCrossRefGoogle Scholar
  23. Lopez, J.R., Wanek, L.A. and Stuart, S.R. (1981). Skeletal muscle: Length dependent effects of potentiating agents. Science 214: 79–82.PubMedCrossRefGoogle Scholar
  24. Orenstein, J., Hogan, D, and Bloom, S. (1980). Surface cables of cardiac myocytes. J. Mol. Cell. Cardiol. 12: 771–780.PubMedCrossRefGoogle Scholar
  25. Podolsky, R.S. and Teicholz, L.E. (1970). The relation between calcium and contraction kinetics in skinned fibers. J. Physiol. 211: 19–35.PubMedGoogle Scholar
  26. Pollack, G.H. and Krueger, J.W. (1976). Sarcomere dynamics of intact cardiac muscle. Eur. J. Cardiol. 4 (Suppl): 53–65.PubMedGoogle Scholar
  27. Taylor, S.R. Decreased activation in skeletal muscle fibres at short lengths. In: The Physiological Basis of Starling’s Lau of the Heart, Ciba Foundation Symposium 24, Associated Scientific Publishers, Amsterdam.Google Scholar
  28. ter Keurs, H.E.D.J., Rijnsburger, W.H., Van Heuningen, R. and Nagelsmit, M. (1980). Tension development and sarcomere length in rat cardiac trabeculae. Circ. Res. 46: 703–714.PubMedCrossRefGoogle Scholar
  29. Whalen, D.A., Martyn, D.A. and Huntsman, L.L. (1981). Passive contributions to load clamp determined segment shortening velocities in isolated ferret papillary muscle. Abstract. Biophys. J. 33: 29a.Google Scholar
  30. Winegrad, S. The importance of passive elements in the contraction of the heart. In: Cardiac Dynamics,J. Baan, A.C. Arntzenius and E.L. Yellin, eds., Martinus Nijhofi Publishers, The HagueGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Donald A. Martyn
    • 1
  • Jeff F. Rondinone
    • 1
  • Lee L. Huntsman
    • 1
  1. 1.Center for Bioengineering WD-12University of WashingtonSeattleUSA

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