Changes in [Ca2+]i Induced by Rapid Cooling of Single Skeletal Muscle Fibres Treated with Low Concentration of Caffeine

  • S. Kurihara
  • M. Konishi
  • T. Sakai
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37)

Abstract

In single skeletal muscle fibres treated with low concentration of caffeine, lowering the bathing solution temperature from 18°C to below 7°C increased [Ca2+]i in three phases depending on caffeine concentration and temperature. Tension could be fully developed (rapid cooling contracture, RCC) by the second phase of the released Ca2+. The second and third phases were inhibited by low concentrations of procaine, and the first phase was blocked by a higher one. RCC was observed even in Ca2+-free solution. The mechanism of [Ca2+]i changes and RCC during cooling was discussed.

Keywords

Caffeine Concentration Single Muscle Fibre Japan Acad Reach Maximum Level Induce Calcium Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allen, D.G. and Blinks, J.R. (1979). The interpretation of light signals from aequorin-injected skeletal and cardiac muscle cells: A new method of calibration. In: Detection and Measurement of Free Ca2+ in Cells. pp. 159–174, ed. Ashley, C.C. and Campbell, A.K. Elsevier/North-Holland.Google Scholar
  2. Blinks, J.R. and Rüdel, R. and Taylor, S.R. (1978). Calcium transients in isolated amphibian skeletal muscle fibres: Detection with aequorin. J. Physiol. 277: 291–323.PubMedGoogle Scholar
  3. Ebashi, S. and Endo, M. (1988). Calcium and muscle contraction. Prog. Biophy. Mol. Biol. 18: 123–183.CrossRefGoogle Scholar
  4. Endo, M. (1975). Mechanism of action of caffeine on the sarcoplasmic reticulum of skeletal muscle. Proc. Japan Acad. 51: 479–484.Google Scholar
  5. Lilttgau, H.C. and Oetliker, H. (1968). The action of caffeine on the activation of the contractile mechanism in striated muscle fibres. J. Physiol., 194: 51–74.Google Scholar
  6. Sakai, T. and Kurihara, S. (1974). A study on rapid cooling contracture from the view point of excitation-contraction coupling. Jikeikai Med. J. 21: 47–88.Google Scholar
  7. Thorens, S. and Endo, M. (1975). Calcium-induced calcium release and “Depolarization”- induced calcium release: their physiological significance. Proc. Japan Acad. 51: 473–478.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • S. Kurihara
    • 1
  • M. Konishi
    • 1
  • T. Sakai
    • 1
  1. 1.Department of PhysiologyThe Jikei University School of MedicineTokyoJapan

Personalised recommendations