An Actomyosin Motor
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 37)
I would like to report some results obtained by Yano, Yamamoto and myself on a novel system (Yano et al., 1982) we have named the actomyosin motor in which a rotor with attached F-actin rotates in a specific direction, driven by the ATP-splitting interaction with active fragments of myosin, heavy meromyosin or subfragment-1, in a solution containing MgATP. The actomyosin motor is not only interesting as a new kind of motor made of biological material but also, as a stream cell (Yano, 1978; Yano et al., 1978; Yano & Shimizu, 1978; Shimizu & Yano, 1978; Shimizu, 1979), is suitable for the study of chemo-mechanical coupling by actin and active fragments of myosin. Active motion of the motor was observed in almost 100% of the experiments, when carefully performed.
KeywordsActin Filament Thin Filament Arrow Head Cyanogen Bromide Active Fragment
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Brown, S.S. and Spudich, J.A (1979). Nucleation of polar actin filament assembly by a positively charged surface. J. Cell. Biol. 80: 499–504.PubMedCrossRefGoogle Scholar
Shimizu, H. (1979). Dynamic cooperativity of molecular processes in active streaming, muscle contraction and subcellular dynamics. Adv. Biophys. 13: 195–278.PubMedGoogle Scholar
Shimizu, H. and Yano, M. (1978). Studies of the chemo-mechanical conversion in artificially produced streamings. M. Dynamic cooperativity. A new cooperativity in actomyosin system with a polarized arrangement of F-actin. J. Biochem. 84: 1093–1102.PubMedGoogle Scholar
Yano, M. (1978). Observation of steady streamings in a solution of Mg-ATP and acto-heavy meromyosin from rabbit skeletal muscle. J. Biochem. 83: 1203–1204.PubMedGoogle Scholar
Yano, M. and Shimizu, H. (1978). Studies of the chemo-mechanical conversion in artificially produced streamings. II. An order-disorder phase transition in the chemo-mechanical conversion. J. Biochem. 84: 1087–1092.PubMedGoogle Scholar
Yano, M., Yamada, T. and Shimizu, H. (1978). Studies of the chemo-mechanical conversion in artificially produced streamings. I. Reconstruction of a chemo-mechanical system from acto-HMM of rabbit skeletal muscle. J. Biochem. 84: 277–283.PubMedGoogle Scholar
Yano, M., Yamamoto, Y. and Shimizu, H. (1982). Actomyosin motor. Nature. In press.Google Scholar
© Plenum Press, New York 1984