31P NMR Studies of Resting Muscle in Normal Human Subjects

  • D. R. Wilkie
  • M. J. Dawson
  • R. H. T. Edwards
  • R. E. Gordon
  • D. Shaw
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37)


Study of human tissues using 31P Topical Magnetic Resonance is completely atraumatic; it allows simultaneous measurement of the concentrations of many important metabolites and of intracellular pH. In some critical situations, TMR yields more accurate results than those obtained by chemical analysis of tissue biopsies. We have shown that TMR can be calibrated to obtain quantitative measurements in human subjects. We have also shown that theories of control of glycolysis based on regulation by key metabolites of rate-limiting enzymes are inconsistent with the observed changes in intact muscle.


Nuclear Magnetic Resonance Needle Biopsy Human Muscle Sensitive Volume Frog Muscle 
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  1. Atkinson, D.E. (1977). In: Cellular Energy Metabolism and its Regulation. Academic Press. New York.Google Scholar
  2. Bendat, J.S. and Piersol, A.G. (1971). In: Random Data Analysis and Measurement Procedures. Wiley-Inter Science, New York. pp. 122–125.Google Scholar
  3. Block, R.J. and Weiss, K.W. (1956). In: Amino Acid Handbook: Methods and Results of Protein Analysis. Charles Thomas. p. 343. Table 5.Google Scholar
  4. Campbell, I.D., Dobson, C.M., Williams, R.J.P. and Xavier, A.V. (1973). Resolution enhancement of protein PMR spectra using the difference between a broadened and a normal spectrum. J. Mag. Res. 11: 172–181.Google Scholar
  5. Chalovich, T.M., Burt, C.T., Danon, M.J., Glonek, T. and Barâny, M. (1979). Phosphodiesters in muscular dystrophies. Ann. N.Y. Acad. Sci. 317: 649–668.Google Scholar
  6. Dawson, M.J., Gadian, D.G. and Wilkie, D.R. (1977). Contraction and recovery of living muscle studied by 31P nuclear magnetic resonance. J. Physiol. 267: 703–735.PubMedGoogle Scholar
  7. Dawson, M.J., Gadian, D.G. and Wilkie, D.R. (1978). Muscular fatigue investigated by phosphorus nuclear magnetic resonance. Nature 274: 861–868.PubMedCrossRefGoogle Scholar
  8. Dawson, M.J., Gadian, D.G. and Wilkie, D.R. (1980). Studies of the biochemistry of contracting and relaxing muscle by the use of 31F n.m.r. in conjunction with other techniques. Phil. Trans. R. Soc. Lond. B289: 445–455.Google Scholar
  9. Dawson, M.J. (1983). Nuclear magnetic resonance. In: Cardiac Metabolism. Ed. A.J. Drake-Holland and M.I.M. Noble. Wiley and Sons Ltd. pp. 309–337.Google Scholar
  10. Diem, K. (1962). Documenta Geigy Scientific Tables. 6th edition. Geigy Pharmaceutical Co. Ltd. Manchester. p. 172.Google Scholar
  11. Edwards, R.H.T., Harris, R.C. and Jones, D.A. (1981). The biochemistry of muscle biopsy in man: clinical applications. Recent Adv. in Clin. Biochem. 2: 243–269.Google Scholar
  12. Edwards, R.H.T., Dawson, M.J., Wilkie, D.R., Gordon, R.E. and Shaw, D. (1982a). Clinical use of nuclear magnetic resonance in the investigation of myopathy Lancet, March 27, 1982, pp. 725–731.Google Scholar
  13. Edwards, R.H.T., Wilkie, D.R., Dawson, M.J., Gordon, R.E. and Shaw, D. (1982b). Measurement of muscle pH and intermediary metabolism by 31P topical magnetic resonance (TMR) in normal subjects and patients with myopathy. European Society for Clinical Investigation. Annual Meeting Luxembourg, 15–17 April, 1982.Google Scholar
  14. Gadian, D.G., Radda, G.K., Brown, T.R., Chance, E.M., Dawson, M.J. and Wilkie, D.R. (1981). The activity of creatine kinase in frog skeletal muscle studied by saturation-transfer nuclear magnetic resonance. Biochem J. 194: 215–228.PubMedGoogle Scholar
  15. Gordon, R.E., Hanley, P.E., Shaw, D., Gadian, D.G., Radda, G.K., Styles, P., Bore, P.J. and Chan, L. (1980). Localization of metabolites in animals using 31P topical magnetic resonance. Nature 287: 736–738.PubMedCrossRefGoogle Scholar
  16. Haljamae, H. and Enger, E. (1975). Human skeletal muscle energy metabolism during and after complete tourniquet ischemia. Ann. Surg. 182: 9–14.Google Scholar
  17. Harris, R.C., Hultman, E, and Nordesjö, L.-O. (1974). Glycogen, glycolytic intermediates and high-energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest. Methods and variance of values. Scand. J. Clin. Lab. Invest. 33: 109–120.Google Scholar
  18. Harris, R.C., Edwards, R.H.T., Hultman, E., Nordesjö, L-O., Nylind, B. and Sahlin, K. (1976). The time course of phosphorylcreatine resynthesis during recovery of the quadriceps muscle in man Pflugers Archiv. 367: 137–142.Google Scholar
  19. Henderson, T.O., Costello, A.J.R. and Omachi, A. (1974). Phosphate metabolism in intact human erythrocytes: determination by phosphorus-31 nuclear magnetic resonance spectroscopy. Proc. Nat. Acad. Sci. 71: 2487–2490.Google Scholar
  20. Huxley, T.H. (1885). Presidential address. Proc. Roy. Soc. 39: 294.Google Scholar
  21. Kastenschmidt, L.L. (1970). The metabolism of muscle as a food. Physiology and Biochemistry of Muscle as a Food. E. Briskey, Cassens, Marsh. Univ. of Wisconsin Press. Vol. 2, pp. 735–753.Google Scholar
  22. Kretzschmar, K.M. and Wilkie, D.R. (1989). A new approach to freezing tissues rapidly. J. Physiol. 202: 66–87 P.Google Scholar
  23. Kretzschmar, K.M. (1970). Energy production and chemical change during muscular contraction. Ph.D. Thesis. Univ. of London. p. 126 and Table 3.Google Scholar
  24. Lehninger, A.L. (1975). Biochemistry. 2nd Ed. Worth Publishers Inc., New York, N.Y. p. 425.Google Scholar
  25. Newsholme, E.A. and Start, C. (1973). Regulation in Metabolism. John Wiley and Sons, London.Google Scholar
  26. Opie, L.H. (1976). II Metabolic regulation in ischemia and hypoxia. Supp. 1. Circ. Res. 38: 1–52 1–174.Google Scholar
  27. Sahlin, K., Harris, R.C. and Hultman, E. (1975). Creatine kinase equilibrium and lactate content compared with muscle pH in tissue samples obtained after isometric exercise. Biochem. J. 152: 173–180.Google Scholar
  28. Sahlin, K., Palmskog, G. and Hultman, E. (1978). Adenine nucleotide and IMP contents of the quadriceps muscle in man after exercise. Pflugers Arch. 374: 193–198Google Scholar
  29. Spector, W.S. Editor (1956). Handbook of Biological Data. Wright Air Development Center Technical Report 56–273. United States Air Force, Wright-Patterson Air Force Base, Ohio.Google Scholar
  30. Stryer, L. (1981). Biochemistry. 2nd ed. W.H. Freeman and Co., San Francisco. p. 299.Google Scholar
  31. Wilkie, D.R. (1981a). Comment At Royal Society Meeting “The Enzymes of Glycolysis: Structure, Activity and Evolution”. Oct. 16, 1980. Phil. Trans. R. Soc. Lond. B293: 40–41.Google Scholar
  32. Wilkie, D.R. (1981b). Shortage of chemical fuel as a cause of fatigue: studies by nuclear magnetic resonance and bicycle ergometry. CIBA Foundation Symposium No. 82, Human Muscle Fatigue: Physiological Mechanisms. Pitman Medical, London.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • D. R. Wilkie
    • 1
  • M. J. Dawson
    • 1
  • R. H. T. Edwards
    • 1
  • R. E. Gordon
    • 2
  • D. Shaw
    • 2
  1. 1.School of MedicineUniversity College LondonLondonEngland
  2. 2.Oxford Research SystemsOxfordEngland

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