Contraction Bands: Differences between Physiologically vs. Maximally Activated Single Heart Muscle Cells

  • John W. Krueger
  • Barry London
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37)

Abstract

High resolution interference and phase microscopy were used to inspect the striations’ appearance in shortening rat heart cells. Isolated cells were treated with detergent so that shortening could be graded by addition of calcium. Upon activation sarcomeres shortened to form (a) contraction densities in the middle of the A band at 1.7 um, (b) disappearance of the I bands and (c) phase brightening of the A bands at 1.8 um, and (d) dense Cz contraction bands at shorter lengths. These changes are totally consistent with the uniform sliding of myofilaments of previously accepted fixed dimensions. However, the striated patterns differed significantly in intact cells which were electrically stimulated to shorten. Here individual A bands remained distinct, without phase brightening or contraction band formation despite sarcomere shortening to less than the length of the A band as measured in the unstimulated cell. Maximal activation of intact cells by barium contracture elicited the full sequence of striation changes (a-d) seen in the chemically skinned cells. Light diffraction analysis gave comparable interpretation, i.e., the protein within the shortened sarcomnere in the physiologically activated cardiac cell is more narrowly distributed than expected for thick filaments of fixed dimensions. These optical differences may reflect the restricted presence of the globular myosin heads at the ends of the cardiac sarcomere. This situation would explain the narrow range of the cardiac length-tension relation.

Keywords

Albumin EDTA Barium Fibril Hepes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anversa, P., Loud, A.V., Giacomelli, F. and Wiener, J. (1978). Absolute morphometric study of myocardial hypertrophy in experimental hypertension. II. Ultrastructure of myocytes and interstitium. Lab. Invest. 38: 597–609.Google Scholar
  2. Brown, L., Gonzalez-Serrates, H. and Huxley, A.F. (1970). Electron microscopy of frog muscle fibre in extreme passive shortening. J. Physiol. 208: 86–88 P.Google Scholar
  3. Fabiato, A. and Fabiato, F. (1976). Dependence of calcium release, tension generation and resting forces on sarcomere length in skinned cardiac cells. Eur. J. Cardiol. 4/suppl. 1327.Google Scholar
  4. Fabiato, A. and Fabiato, F. (1979). Calculator programs for computing the composition of the solutions containing multiple metals and liquids used for experiments in skinned muscle cells. J. Physiol. Paris 75: 463–505.PubMedGoogle Scholar
  5. Fujime, S. (1975). Optical diffraction study of muscle fibres. Biochim. Biophys. Acta 3799: 227–238.Google Scholar
  6. Dewey, M.M., Levine, R.J.C., Colflesh, D., Walcott, B., Brann, L., Baldwin, A. and Brink, P. (1979). Structural changes in thicck filaments during sarcomere shortening in Limulus striated muscle. In: Cross-Bridge Mechanism in. Muscle Contraction, 3–19, eds. Sugi, H. and Pollack, G.H., Baltimore: Univ. Park Press.Google Scholar
  7. Gordon, A.M., Huxley, A.F. and Julian, F. (1966). The variation in isometric tension with sarcomere length in vertebrate muscle fibres. J. Physiol. 184: 170–192.PubMedGoogle Scholar
  8. Hasselbach, W., Somer, J.R. and v.Graff, H. (1975). A-band shortening in contracted skeletal muscle fibrils. Fed. Proc. 34: 474.Google Scholar
  9. Haworth, R., Hunter, D.R. and Berkoff, H.A. (1980). The isolation of Caz+ resistant myocytes from the adult rat. J. Molec. Cell Cardiol. 12: 715–724.CrossRefGoogle Scholar
  10. Herman, L. and Dreizen, P. (1971). Electron microscopic studies of skeletal and cardiac muscle of a benthic fish. I. Myofibrillar structure in resting and contracted muscle. Am. Zool. 11: 543–557.Google Scholar
  11. Hill, L. (1977). A-band length, striation spacing and tension change on stretch of active muscle. J. Physiol. 226: 677–685.Google Scholar
  12. Huxley, A.F. and Gordon, A.M. (1962). Striation patterns in active and passive shortening of muscle. Nature 193: 280–281.PubMedCrossRefGoogle Scholar
  13. Huxley, A.F. and Niedergerke, R. (1954). Structural changes in muscle during contraction: Interference microscopy of living muscle fibres. Nature 173: 971–973.PubMedCrossRefGoogle Scholar
  14. Huxley, A.F. and Niedergerke, R. (1958). Measurement of the striations of isolated muscle fibres with the interference microscope. J. Physiol. 144: 403–441.PubMedGoogle Scholar
  15. Huxley, H.E. and Hanson, J. (1954). Changes in the cross striations of muscle during contraction and stretch and their structural interpretation. Nature 173: 973–976.PubMedCrossRefGoogle Scholar
  16. Krueger, J.W., Forletti, D. and Wittenberg, B.A. (1980). Uniform sarcomere shortening behavior in isolated cardiac muscle cells. J. Gen. Physiol. 76: 587–607.PubMedCrossRefGoogle Scholar
  17. Page, S.G. (1974). Measurement of structural parameter in cardiac muscle. CIBA Foundation Symposium 24: 11–26, Elsevier, Amsterdam.Google Scholar
  18. Pepe, F.A. (1967). The myosin filament II. Interaction between myosin and active filament observed using antibody staining in fluorescent and electron microscopy. J. Molec. Biol. 27: 227–236.CrossRefGoogle Scholar
  19. Pollack, G.H. and Krueger, J.W. (1976). Sarcomere dynamics in intact cardiac muscle. Eur. J. Cardiol. 4/suppl., 53–65.PubMedGoogle Scholar
  20. Rüdel, R. and Zite-Ferenczi, F. (1980). Efficiency of light diffraction by cross striated muscle fibers under stretch and during isometric contraction. Biophys. J. 30: 507–516.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • John W. Krueger
    • 1
  • Barry London
    • 1
  1. 1.Depts. of Medicine and Physiology/BiophysicsThe Albert Einstein College of MedicineBronxUSA

Personalised recommendations