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The Trace Determination of Amineptine and Its Main Metabolite by HPLC Application to Amineptine Plasma Levels Following Oral Administration in Humans

  • G. Nicot
  • G. Lachatre
  • J. P. Valette
  • L. Merle
  • Y. Nouaille
  • N. Bromet
  • E. Mocaer

Abstract

An isocratic reversed-phase ion-pair liquid chromatographic method was used to determine amineptine and its main metabolite plasma levels. The main parameters of the analytical method were discussed.

Results obtained by this HPLC procedure were compared to those obtained by a GC/MS as reference method. The method was applied to the determination of the pharmacokinetic profile of the drug and its main metabolite during the clinical evaluation of amineptine in patients after oral administration. The sensitivity of the method will allow to follow plasma amineptine and its metabolite in human studies in the range 10 to 1000 ng/ml.

Keywords

Main Metabolite Octanoic Acid Internal Standard Solution Peak Height Ratio Trace Determination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • G. Nicot
    • 1
  • G. Lachatre
    • 1
  • J. P. Valette
    • 1
  • L. Merle
    • 1
  • Y. Nouaille
    • 1
  • N. Bromet
    • 2
  • E. Mocaer
    • 2
  1. 1.Service de PharmacologieClinique CHRU DupuytrenLimogesFrance
  2. 2.Institut de Recherches InternationalesSERVIERNeuilly-sur-SeineFrance

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