Correction of Genetic Gonadotropic Hormone-Releasing Hormone Deficiency by Preoptic Area Transplants

  • Dorothy T. Krieger
  • Marie J. Gibson


The hypogonadal (hpg) mutant mouse appeared in a breeding colony at Harwell, England. The mutant mice of both sexes fail to show any sign of postnatal gonadal or accessory sexual tissue development. However, sexual differentiation proceeds normally up to birth. Hormone assays and immunoperoxidase staining indicate that the primary cause of the hypogonadism is due to a severe deficiency in the hypothalamic releasing hormone [gonadotropin-releasing hormone (GnRH)] that governs pituitary luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release. In view of previous studies demonstrating that transplants of embryonic mammalian CNS tissue survive, differentiate, and become anatomically integrated with the CNS of neonatal and adult mammalian recipients,1–16 we were interested to see if transplants of normal fetal tissue could correct this genetically induced CNS defect. For the transplant material, we used fetal preoptic area (POA), a primary site of GnRH cell bodies. To more fully understand the nature of the results obtained, it is necessary to first briefly review the normal regulation of the hypothalamic—pituitary—gonadal axis and the details of the nature of the gonadal deficiency of the hpg animal.


Luteinizing Hormone Median Eminence Preoptic Area GnRH Neuron Luteinizing Hormone Release 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Dorothy T. Krieger
    • 1
  • Marie J. Gibson
    • 1
  1. 1.Division of EndocrinologyMount Sinai School of MedicineNew YorkUSA

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