The Molecular Biologists Attack the Problem
Studies on the nucleic acids involved in immunoglobulin synthesis had been underway for over a decade when the major breakthroughs came with the application of recombinant DNA technology to the antibody problem in the late 1970s. The essential groundwork for these break-throughs had been laid by the discovery of the mouse myelomas by Potter and their adaptation to tissue culture. The new wave of discovery would be further based on the early work by Askonas, Williamson, Stavitsky, Scharff, Milstein, Mach, and others who isolated mRNA from these tumors and developed techniques for its purification and enrichment as well as assays for the synthesis in vitro of immunoglobulin polypeptide chains. The first decade of work on nucleic acids was not particularly fruitful because the powerful technologies that emerged in the mid 1970s and were immediately applied to the antibody problem had not yet been developed.
KeywordsLight Chain Heavy Chain Gene Segment Molecular Biologist Immunoglobulin Gene
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