The problem of antibody diversity was of great concern to immunologists, and therefore it was appropriate that they study this problem with the serologic methods with which they were familiar. In retrospect, this was a fortuitous circumstance that allowed a rapid initial burst of information on this subject. At the time these serologic studies were begun, techniques for protein structure determination were not sufficiently developed to handle large complex molecules, nor were there available procedures for protein isolation suitable for the mixtures of molecules contained in most antibody populations. Indeed, the concept that a single molecule might have multiple, disulfide-linked subunits was years away. Even more remote was the idea of directly studying the genes encoding antibodies. Serology, on the other hand, was highly developed by the early 1950s. The fact that antibodies with exquisite specificity could be produced by immunization was fully appreciated. An impressive list of scientific pioneers including Pasteur, Koch, Von Behring, Erhlich, and Landsteiner had already contributed to this area.
KeywordsLight Chain Heavy Chain Versus Region Cold Agglutinin Myeloma Protein
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