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Production of Human Monoclonal Antibodies

  • David W. Buck
  • James W. Larrick
  • Andrew Raubitschek
  • Kenneth E. Truitt
  • G. Senyk
  • J. Wang
  • Bradley J. Dyer

Abstract

The need to obtain human monoclonal antibodies has not been overshadowed by the immense success of mouse monoclonal antibody technology. Human monoclonal antibodies will be less antigenic for human in vivo studies and therapy; they will be more likely to recognize antigenic subtleties not easily detected by xenogeneic antibodies, and they will have less rapid catabolism in vivo. The study of human B-cell differentiation and development will be furthered by the availability of human hybridomas. These hybridomas also will be useful to dissect the human humoral immune response in autoimmune disease, cancer, and allergy.

Keywords

Human Monoclonal Antibody Tetanus Toxoid Lymphoblastoid Cell Line Keyhole Limpet Hemocyanin Human Immunoglobulin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aldo-Benson, M., and Scheiderer, L., 1983, Long term growth of lines of murine dinitrophenyl-specific B lymphocytes in vitro, J. Exp. Med. 157:342–347.CrossRefGoogle Scholar
  2. Bengtsson, B. O., Nabholz, M., Kennett, R. H., and Bodmer, W. F., 1975, A genetic and karyotypic analysis of crosses between lymphocytes and D98/AH-2, Somatic Cell Genet. 1:41–64.CrossRefGoogle Scholar
  3. Boylston, A. W., Gardner, B., Anderson, R. L., and Hughes-Jones, W. C., 1980, Production of human IgM anti-D in tissue culture by EB-virus transformed lymphocytes, Scand. J. Immunol. 12:355–358.CrossRefGoogle Scholar
  4. Brahe, C., and Serra, A., 1981, A simple method for fusing human lymphocytes with rodent cells in monolayer by polyethylene glycol, Somatic Cell Genet. 7:109–115.CrossRefGoogle Scholar
  5. Brown, N. A., and Miller, G., 1982, Immunoglobulin expression by human B lymphocytes clonally transformed by Epstein-Barr virus, J. Immunol. 128:24–29.Google Scholar
  6. Burk, K. M., Drewinko, B., Trujillo, J. M., and Ahearn, M. J., 1978, Establishment of a human plasma cell line in vitro, Cancer Res. 38:2508–2513.Google Scholar
  7. Butler, J. L., Lane, H. C., and Fauci, A. S., 1983a, Delineation of optimal conditions for producing mouse/human heterohybridomas from human peripheral blood B cells of immunized subjects, J. Immunol. 130:165–168.Google Scholar
  8. Butler, J. L., Muraguchi, A., Lane, H. C., and Fauci, A. S., 1983b, Development of a human T-T cell hybridoma secreting B cell growth factor, J. Exp. Med. 157:60–68.CrossRefGoogle Scholar
  9. Buttin, G., LeGuern, G., Phalente, L., Lin, E. C. C., Medrano, L., and Cazenave, P. A., 1978, Production of hybrid lines secreting monoclonal anti-idiotypic antibodies by cell fusion on membrane filters, in: Lymphocyte Hybridomas (F. Melchers, M. Potter, and N. I. Warner, eds.), Springer-Verlag, Berlin, pp. 27–36.Google Scholar
  10. Callard, R., McCaughan, G., Babbage, J., and Souhami, R., 1982, Specific in vitro antibody responses by human blood lymphocytes: Apparent nonresponsiveness of PBL is due to a lack of recirculating memory B cells, J. Immunol. 129:153–156.Google Scholar
  11. Cavagnaro, J., and Osband, M., 1983, Successful in vitro primary immunization of human peripheral blood mononuclear cells and its role in the development of human derived monoclonal antibodies, Biotechniques 1:31–36.Google Scholar
  12. Chiorazzi, N., Wasserman, R. L., and Kunkel, H. G., 1982, Use of Epstein-Barr virus transformed B cell lines for the generation of immunoglobulin-producing human B cell hybridomas, J. Exp. Med. 156:930–935.CrossRefGoogle Scholar
  13. Cote, R. J., Morrissey, D. M., Houghton, A. N., Beattie, Jr., E. J., Oettgen, H. F., and Old, L. J., 1983, Generation of human monoclonal antibodies reactive with cellular antigens, Proc. Natl. Acad. Sci. USA 80:2026–2030.CrossRefGoogle Scholar
  14. Cotton, R. G. H., and Milstein, C., 1973, Fusion of two immunoglobulin producing myeloma cells, Nature 244:42–43.CrossRefGoogle Scholar
  15. Crawford, D. H., Barlow, M. J., Harrison, J. F., Winger, L., and Huehns, E. R., 1983a, Production of human monoclonal antibody to rhesus D antigen, Lancet 1983 (February 19):386–388.CrossRefGoogle Scholar
  16. Crawford, D., Callard, R. E., Muggeridge, M. I., Mitchell, D. M., Zanders, E. D., and Beverley, P. C. L., 1983b, Production of human monoclonal antibody to X31 influenza virus nucleoprotein, J. Gen. Virol. 64:697–700.CrossRefGoogle Scholar
  17. Croce, C. M., Shander, M., Martinis, J., Cicurel, L., D’Ancona, G. G., Dolby, T. W., and Koprowski, H., 1979, Chromosomal location of the genes for human immunoglobulin heavy chains, Proc. Natl. Acad. Sci. USA 76:3416–3419.CrossRefGoogle Scholar
  18. Croce, C. M., Linnenbach, A., Hall, W., Steplewski, Z., and Koprowski, H., 1980, Production of human hybridomas secreting antibodies to measles virus, Nature 288:488–489.CrossRefGoogle Scholar
  19. Dalla-Favera, R., Breeni, M., Erikson, J., Patterson, D., Gallo, R. C., and Croce, C., 1982, Human c-myc oncgene is located on the region of chromosome 8 that is translocated in Burkitt’s lymphoma cells, Proc. Natl. Acad. Sci. USA 79:7824–7827.CrossRefGoogle Scholar
  20. Dwyer, D. S., Bradley, R. J., Urguhart, C. K., and Kearney, J. F., 1983, Naturally occurring anti-idiotypic antibodies in myasthenia gravis patients, Nature 301:611–614.CrossRefGoogle Scholar
  21. Edwards, P. A. W., Smith, C. M., Neville, A. M., and O’Hare, M. J., 1982, A human/human hybridoma system based on a fast growing mutant of the ARH-77 plasma cell leukemia derived line, Eur. J. Immunol. 12:641–648.CrossRefGoogle Scholar
  22. Eisenbarth, G. S., Linnenbach, A., Jackson R., Scearce R., and Croce, C. M., 1982, Human hybridomas secreting anti-islet antibodies, Nature 300:264–267.CrossRefGoogle Scholar
  23. Ellison, J., and Hood, L., 1982, Linkage and sequence homology of two human immunoglobulin gamma heavy chain constant region genes, Proc. Natl. Acad. Sci. USA 79:1984–1988.CrossRefGoogle Scholar
  24. Erikson, J., Martinis, J., and Croce, C. M., 1981, Assignment of the genes for human immunoglobulin chains to chromosome 22, Nature 294:173–175.CrossRefGoogle Scholar
  25. Foung, S. K. H., Sasaki, D., Grumet, F. C., and Engleman, E. G., 1982, Production of functional human T/T hybridomas in selection medium lacking aminopterin and thymidine, Proc. Natl. Acad. Sci. USA 79:7484–7488.CrossRefGoogle Scholar
  26. Gigliotti, F., and Insel, R. A., 1982, Protective human hybridoma antibody to tetanus toxin, J. Clin. Invest. 70:1306–1309.CrossRefGoogle Scholar
  27. Glassy, M. C., Handley, H. H., Royston, I., and Lowe, D. H., 1983, Human monoclonal antibodies to human cancers, in: Proceeding of the 4th Arm and Hammer Cancer Symposium (B. D. Boss, R. Langman, I. S. Trowbridge, and R. Dulbecco, eds.), Academic Press, New York, pp. 163–170.Google Scholar
  28. Goodman, M., and Weigel, W., 1983, Activation of lymphocytes by a thiol-derivatized nucleoside: Characterization of cellular parameters and responsive subpopulations, J. Immunol. 130:552–557.Google Scholar
  29. Gronowicz, E., Coutinho, A., and Melchers, F., 1976, A plaque assay for all cells secreting Ig of a given type or class, Eur. J. Immunol. 6:588–590.CrossRefGoogle Scholar
  30. Handley, H. H., and Royston, L, 1982, A human lymphoblastoid B cell line useful for generating immunoglobulin secreting human hybridomas, in: Hybridomas in Cancer Diagnosis and Treatment (M. S. Mitchell and H. F. Oettgen, eds.), Raven Press, New York, pp. 125–132.Google Scholar
  31. Handley, H., Royston, I., and Glassy, M. C., 1983, The production of human monoclonal antibodies to human tumor associated antigens, in: Proceedings of the 15th International Leucocyte Conference (J. W. Parker and R. L. O’Brien, eds.), Wiley Interscience, New York, pp. 617–620.Google Scholar
  32. Heiter, P. A., Max, E. E., Maizel, J. V., and Leder, P., 1980, Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments, Cell 22:197–207.CrossRefGoogle Scholar
  33. Heiter, P. A., Hollis, G. F., Korsmeyer, S. J., Waldman, T. A., and Leder, P., 1981, Clustered arrangement of immunoglobulin lambda constant region genes in man, Nature 294:536–540.CrossRefGoogle Scholar
  34. Heiter, P. A., Korsmeyer, S. J., Waldman, T. A., and Leder, P., 1981, Human immunoglobulin k light-chain genes are deleted or rearranged in X-producing B cells, Nature 290:368–372.CrossRefGoogle Scholar
  35. Hirohashi, S., and Shimosato, Y., 1982, In vitro production of tumor-related human monoclonal antibody and its immunohistochemical screening with autologous tissue, Gann 73:345–347.Google Scholar
  36. Hoffman, M., 1980, Antigen-specific induction and regulation of antibody synthesis in cultures of human peripheral blood mono-nuclear cells, Proc. Natl. Acad. Sci. USA 77:1139–1143.CrossRefGoogle Scholar
  37. Houghton, A. N., Brooks, H., Cote, R. J., Taormina, M. C., Oettgen, H. F., and Old, L. J., 1983, Detection of cell surface and intracellular antigens by human monoclonal antibodies: Hybrid cell lines derived from lymphocytes of patients with malignant melanoma, J. Exp. Med. 158:53–65.CrossRefGoogle Scholar
  38. Howard, M., Kessler, S., Chused, T., and Paul W., 1981, Long term culture of normal mouse B lymphocytes, Proc. Natl. Acad. Sci. USA 78:5788–5792.CrossRefGoogle Scholar
  39. Howard, M., Farrar, J., Hilfiker, M., Johnson, B., Takatsu, K., Hamaoka, T., and Paul, W., 1982, Identification of a T cell-derived B cell growth factor distinct from interleukin-2, J. Exp. Med. 155:914–923.CrossRefGoogle Scholar
  40. Irie, R. F., Sze, L. L., and Saxton, R. E., 1982, Human antibody to OFA-1, a tumor antigen produced in vitro by Epstein-Barr virus-transformed human B-lymphoid cell lines, Proc. Natl. Acad. Sci. USA 79:5666–5670.CrossRefGoogle Scholar
  41. Kamo, I., Furukawa, S., Tada, A., Mano, Y., Iwasaki, Y., and Furuse, T., 1982, Monoclonal antibody to acetylcholine receptor: Cell line established from thymus of patient with myasthenia gravis, Science 215:995–997.CrossRefGoogle Scholar
  42. Karpas, A., Fischer, P., and Swirsky, D., 1982, Human myeloma cell line carrying a Philadelphia chromosome, Science 216:997–999.CrossRefGoogle Scholar
  43. Kearney, J. F., Radbruch, A., Liesegang, B., and Rajewsky, K., 1979, A new mouse myeloma line that has lost immunoglobulin expression that permits the construction of antibody secreting hybrid cell lines, J. Immunol. 123:1548–1550.Google Scholar
  44. Kohler, G., and Milstein, C., 1975, Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256:495–497.CrossRefGoogle Scholar
  45. Kohler, G., and Shulman, M. J. L., 1978, Cellular and molecular restrictions of the lymphocyte fusion, in: Lymphocyte Hybridomas (F. Melchers, M. Potter, and N. I. Warner, eds.), Springer-Verlag, Berlin, pp. 143–148.Google Scholar
  46. Kohler, G., Howe, C. S., and Milstein, C., 1976, Fusion between immunoglobulin secreting and non-secreting lines, Eur. J. Immunol. 6:292–295.CrossRefGoogle Scholar
  47. Korsmeyer, S. J., Hieter, P., Ravetch, J. V., Poplack, D. G., Waldmann, T. A., and Leder, P., 1981, Developmental hierarchy of immunoglobulin gene rearrangements in human leukemic pre-B-cells, Proc. Natl. Acad. Sci. USA 78:7096–7100.CrossRefGoogle Scholar
  48. Koskimies, S., 1980, Human lymphoblastoid cell line producing specific antibody against Rh-antigen D, Scand. J. Immunol. 11:73–77.CrossRefGoogle Scholar
  49. Kozbor, D., and Roder, J., 1981, Requirements for the establishment of high titred human monoclonal antibodies against tetanus toxoid using the Epstein-Barr virus technique, J. Immunol. 127:1275–1280.Google Scholar
  50. Kozbor, D., and Roder, J. C., 1983, In vitro stimulated lymphoocytes as a source of human hybridomas, Eur. J. Immunol., in press.Google Scholar
  51. Kozbor, D., Steinitz, M., Klein, G., Koskimies, S., and Makela, O., 1979, Establishment of anti-TNP antibody-producing human lymphoid lines by preselection for hapten binding followed by EBV transformation, Scand. J. Immunol. 10:187–194.CrossRefGoogle Scholar
  52. Kozbor, D., Lagarde, A. E., and Roder, J. C., 1982a, Human hybridomas constructed with antigen-specific EBV transformed lines, Proc. Natl. Acad. Sci. USA 79:6651–6655.CrossRefGoogle Scholar
  53. Kozbor, D., Roder, J. C., Chang, T. H., Steplewski, Z., and Koprowski, H., 1982b, Human antitetanus toxoid monoclonal antibody secreted by EBV-transformed human B cells fused with murine myeloma, Hybridoma 1(3):323–328.CrossRefGoogle Scholar
  54. Kozbor, D., Dexter, D., and Roder, J. C., 1983, A comparative analysis of the phenotypic characteristics of available fusion partners for the construction of human hybridomas, Hybridoma 2(1):7–16.CrossRefGoogle Scholar
  55. Lane, H. C., Shelhamer, J. H., Mostowski, H. S., and Fauci, A. S., 1982, Human monoclonal anti-KLH antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a KLH-immune individual, J. Exp. Med. 155:333–338.CrossRefGoogle Scholar
  56. Larrick, J. W., Truitt, K. E., Raubitschek, A. A., Senyk, G. S., Wang, J. C. N., 1983, Characterization of human hybridomas secreting antibody to tetanus toxoid, Proc. Natl. Acad. Sci. USA 80:6376–6380.CrossRefGoogle Scholar
  57. Lever, J. E., Nuki, G., and Seegmiller, J. E., 1974, Expression of purine overproduction in a series of 8-azaguanine resistant diploid human lymphoblast lines, Proc. Natl. Acad. Sci. USA 71:2679–2683.CrossRefGoogle Scholar
  58. Levy, J. A., Virolainen, M., and Defendi, V., 1968, Human lymphoblastoid lines from lymph node and spleen, Cancer 22:517–524.CrossRefGoogle Scholar
  59. Lewin, R., 1981, An experiment that had to succeed, Science 212:767–769.CrossRefGoogle Scholar
  60. Littlefield, J. W., 1964, Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants, Science 145:709–710.CrossRefGoogle Scholar
  61. Loken, M. R., and Stall, A. M., 1982, Flow cytometry as an analytical and preparative tool in immunology, J. Immunol. Methods 50:R85–R112.CrossRefGoogle Scholar
  62. Madsen, M., and Johnson, H. E., 1979, A methodological study of E-rosette formation using AET treated sheep red blood cells, J. Immunol. Methods 27:61–74.CrossRefGoogle Scholar
  63. Maizel, A., Sahasrabuddhe, C., Mehta, S., Morgan, J., Lachman, L., and Ford, R., 1982, Biochemical separation of a human B cell mitogenic factor, Proc. Natl. Acad. Sci. USA 79:5998–6002.CrossRefGoogle Scholar
  64. Malcom, S., Barton, P., Murphy, C., Ferguson-Smith, M. A., Bentley, D. L., and Rabbitts, T. H., 1982, Localization of human immunoglobulin K light chain variable region genes to the short arm of chromosome 2 by in situ hybridization, Proc. Natl. Acad. Sci. USA 79:4957–4961.CrossRefGoogle Scholar
  65. Matsuoka, Y., Moore, G. E., Yagi, Y., and Pressman, D., 1967, Production of free light chains of immunoglobulin by a haematopoietic cell line derived from a patient with multiple myeloma, Exp. Biol. N. Y. 125:1246–1250.Google Scholar
  66. Matthyssens, G., and Rabbitts, T. H., 1980, Structure and multiplicity of human immunoglobulin heavy chain variable region genes, Proc. Natl. Acad. Sci. USA 77:6561–6565.CrossRefGoogle Scholar
  67. Miller, G., and Lipman, M., 1973, Release of infectious Epstein-Barr virus by transformed marmoset leukocytes, Proc. Natl. Acad. Sci. USA 70:190–194.CrossRefGoogle Scholar
  68. Miller, G., Grogan, E., Heston, H., Robinson, J., and Smith, D., 1981, Epstein-Barr viral DNA: Infectivity for human placental cells, Science 212:452–455.CrossRefGoogle Scholar
  69. Mishell, R., and Dutton, R., 1967, Immunization of dissociated spleen cells cultures from normal mice, J. Exp. Med. 126:424–442.CrossRefGoogle Scholar
  70. Morimoto, C., Reinherz, E., and Schlossman, S., 1981, Regulation of in vitro primary anti-DNP antibody production by functional subsets of T lymphocytes in man, J. Immunol. 127:69–73.Google Scholar
  71. Muraguchi, A., and Fauci, A., 1982, Proliferative responses of normal human B lymphocytes: Development of an assay system for human B cell growth factor (BCGF), J. Immunol. 129:1104–1108.Google Scholar
  72. Muraguchi, A., Butler, J., Kehrl, J., and Fauci, A., (1983), Differential sensitivity of human B cell subsets to activation signals delivered by anti-µ antibody and proliferative signals delivered by a monoclonal B cell growth factor, J. Exp. Med. 157:530–546.CrossRefGoogle Scholar
  73. Nilsson, K., Bennich, H., Johansson, S. G. O., and Ponten, J., 1970, Established immunoglobulin producing myeloma (IgE), Clin. Exp. Immunol. 7:477–489.Google Scholar
  74. Nishida, Y., Miki, T., Hisajima, H., and Honjo, T., 1982, Cloning of human immunoglobulin epsilon chain genes: Evidence for multiple C epsilon genes, Proc. Natl. Acad. Sci. USA 79:3833–3837.CrossRefGoogle Scholar
  75. Nowinski, R., Berglund, C., Lane, J., Lostrom, M., Bernstein, L, Young, W., and Hakomori, S., 1980, Human monoclonal antibody against Forssman antigen, Science 210:537–539.CrossRefGoogle Scholar
  76. Ochi, A., Hawley, R. G., Shulman, M. J., and Hoyuni, N-, 1983, Transfer of a cloned immunoglobulin light chain gene to mutant hybridoma cells restores specific antibody production, Nature 302: 340–342.CrossRefGoogle Scholar
  77. Oi, V. T., Morrison, S. L., Herzenberg, L. A., and Berg, P., 1983, Immunoglobulin gene expression in transformed lymphoid cells, Proc. Natl. Acad. Sci. USA 80:825–829.CrossRefGoogle Scholar
  78. Okada, M., Sakaguchi, N., Yoshimura, N., Hara, H., Shimizu, K., Yoshida, W, Shizaki, K., Kishimoto, S., Yamamura, Y., and Kishimoto, T., 1983, B cell growth factors and B cell differentiation factor from human T hybridomas, J. Exp. Med. 157:538–590.CrossRefGoogle Scholar
  79. Olsson, L., and Kaplan, H. S., 1980, Human/human hybridomas producing monoclonal antibodies of predefined antigenic specificity, Proc. Natl. Acad. Sci. USA 77:5429–5431.CrossRefGoogle Scholar
  80. Potter, M., Humphrey, J. G., and Walters, J. L., 1972, Growth of primary plasmacytomas in the mineral oil conditioned periotoneal environment, J. Natl. Cancer Inst. 49:305–308.Google Scholar
  81. Rabbitts, T. H., Bentley, D. L., and Milstein, C. P., 1981, Human antibody genes: V gene variability and CH gene switching strategies, Immunol. Rev. 59:69–91.CrossRefGoogle Scholar
  82. Ralph, P., and Kishimoto, T., 1981, Tumor promoter phorbol myristir oretak stimulates immunoglobulin secretion correlated with growth cessation in human B lymphocyte cell lines, J. Clin. Invest. 68:1093–1096.CrossRefGoogle Scholar
  83. Ravetch, J. V., Siebenlist, U., Korsmeyer, S., Waldmann, T., and Leder, P., 1981, Structure of the human immunoglobulin µ locus: Characterization of embryonic and rearranged J and D genes, Cell 27:583–591.CrossRefGoogle Scholar
  84. Reedman, B., and Klein, G., 1973, Cellular localization of an Epstein-Barr virus (EBV)-associated complement fixing antigen in producer and non-producer lymphoblastoid cell lines, Int. J. Cancer 11:499–520.CrossRefGoogle Scholar
  85. Rosen, A. S., Britton, B., Gergely, P., Jondal, M., and Klein, G., 1977, Polyclonal Ig production after Epstein-Barr virus infection of human lymphocytes in vitro, Nature 267:52–54.CrossRefGoogle Scholar
  86. Ruddle, F. H., 1974, Human genetic linkage and gene mapping by somatic cell genetics, in: Somatic Cell Hybridization (R. L. Davidson and F. F. de la Cruz, eds.), Raven Press, New York, pp. 1–12.Google Scholar
  87. Sato, K., Slesinski, R. S., and Littlefield, J. W., 1972, Chemical mutagenesis at the phosphoribosyltransferase locus in cultured human lymphoblasts, Proc. Natl. Acad. Sci. USA 69:1244–1248.CrossRefGoogle Scholar
  88. Schlom, J., Wunderlich, D., and Teramoto, Y. A., 1980, Generation of monoclonal antibodies reactive with human mammary carcinoma cells, Proc. Natl. Acad. Sci. USA 77:6841–6845.CrossRefGoogle Scholar
  89. Schoenfeld, Y., Hsu-Lin, S., Gabriels, J., Silberstein, L., Furie, B. C., Furie, B., Stollar, B., and Schwartz, R., 1982, Production of autoantibodies by human-human hybridomas, J. Clin. Invest. 70:205–208.CrossRefGoogle Scholar
  90. Schoenfeld, Y., Rauch, J., Massicotte, H., Datta, S., Schwartz, J., Stollar, D., and Schwartz, R. S., 1983, Polyspecificity of monoclonal lupus autoantibodies produced by human/human hybridomas, New Engl. J. Med. 308(8):414–420.CrossRefGoogle Scholar
  91. Schwaber, J., and Cohen, E. P., 1973, Human/mouse somatic cell hybrid clone secreting immunoglobulin of both parental types, Nature 244:444–447.CrossRefGoogle Scholar
  92. Sikora, K., and Phillips, J., 1981, Human monoclonal antibodies to glioma cells, Br. J. Cancer 43:105–107.CrossRefGoogle Scholar
  93. Sikora, K., and Wright, R., 1981, Human monoclonal antibodies to lung cancer antigens, Br. J. Cancer 43:696–700.CrossRefGoogle Scholar
  94. Sikora, K., Alderton, T., Phillips, J., and Watson, J., 1982, Human hybridomas from malignant gliomas, Lancet i:11.CrossRefGoogle Scholar
  95. Sredni, B., Sieckmann, D., Kumagai, S., House, S., Green, I., and Paul, W., 1981, Long term culture and cloning of nontransformed human B lymphocytes, J. Exp. Med. 154:1500–1516.CrossRefGoogle Scholar
  96. Stanbridge, E. J., Der, C. J., Doerson, C. J., Nishimi, R. Y., Peehl, D. M., Weissman, B. E., and Wilkinson, J. E., 1982, Human cell hybrids: Analysis of transformation and tumorigenicity, Science 215:252–259.CrossRefGoogle Scholar
  97. Stashenko, P., Nadler, L. M., Hardy, R., and Schlossman, S. F., 1980, Characterization of a human B lymphocyte-specific antigen, J. Immunol 125:1678–1685.Google Scholar
  98. Steinitz, M., 1981, Human monoclonal antibodies produced by Epstein-Barr virus-immortalized cell lines, in: Monoclonal Antibodies and T-Cell Hybridomas Perspectives and Technical Advances (G. J. Hammerling, U. Hammerling, and J. F. Kearney, eds.), Elsevier/North-Holland Biomedical Press, New York, pp. 447–452.Google Scholar
  99. Steinitz, M., and Tamir, S., 1982, Human monoclonal autoimmune antibody produced in vitro: Rheumatoid factor generated by Epstein-Barr virus-transformed cell line, Eur. J. Immunol. 12:126–133.CrossRefGoogle Scholar
  100. Steinitz, M., Klein, G., Koskimies, S., and Makela, O., 1977, EB virus-induced B lymphocyte cell lines producing specific antibody, Nature 269:420–22.CrossRefGoogle Scholar
  101. Steinitz, M., Koskimies, S., Klein, G., and Makela, O., 1979, Establishment of specific antibody producing human lines by antigen preselection and Epstein-Barr virus (EBV) transformation, J. Clin. Lab. Immunol. 2:1–7.Google Scholar
  102. Taylor, I. W., and Milthorpe, B. K., 1980, An evaluation of DNA fluorochrome, staining techniques, and analysis for flow cytometry, J. Histochem. Cytochem. 28:1224–1232.CrossRefGoogle Scholar
  103. Togawa, A., Inoue, N., Myamoto, K., Hyodo, H., and Namba, M., 1982, Establishment and characterization of a human myeloma cell line, Int. J. Cancer 29:495–500.CrossRefGoogle Scholar
  104. Tonegawa, S., 1983, Somatic generation of antibody diversity, Nature 302:575–581.CrossRefGoogle Scholar
  105. Tsuchiya, S., Yokoyama, S., Yoshie, O., and Ono, Y., 1980, Production of diphtheria antitoxin antibody in Epstein-Barr virus induced lymphoblastoid cell lines, J. Immunol. 124:1970–1976.Google Scholar
  106. Wetzel, G. D., Swain, S. L., and Dutton, R. W., 1982, A monoclonal T cell-replacing activity can act directly on B cells to enhance clonal expansion, J. Exp. Med. 156:306–311.CrossRefGoogle Scholar
  107. Whitlock, C. A., and Witte, O. N., 1982, Long-term culture of B lymphocytes and their precursors from murine bone marrow, Proc. Natl. Acad. Sci. USA 79:3608–3612.CrossRefGoogle Scholar
  108. Zurawski, V., Black, P., and Haber, E., 1980, Continuously proliferating human cell lines synthesizing antibody of predetermined specificity, in: Monoclonal Antibodies. Hybridomas: A New Dimension in Biological Analyses (R. H. Kennett, T. J. McKearn, and K. Bechtol, eds.), Plenum Press, New York, pp. 19–33.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • David W. Buck
    • 1
  • James W. Larrick
    • 1
  • Andrew Raubitschek
    • 1
  • Kenneth E. Truitt
    • 1
  • G. Senyk
    • 1
  • J. Wang
    • 1
  • Bradley J. Dyer
    • 1
  1. 1.Cetus Immune Research LaboratoriesPalo AltoUSA

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