Reflections on Nine Years of Monoclonal Antibodies from Hybridomas
  • Roger H. Kennett
  • Kathleen B. Bechtol
  • Thomas J. McKearn


It has been 9 years since Kohler and Milstein (1975) first reported the production of monoclonal antibodies with predefined specificity. During the intervening time, these reagents have become valuable tools in biomedical research, some have been given FDA approval, and they have been used on patients for therapy; moreover, recent biotechnology newsletters indicate that the first “over-the-counter” monoclonal antibody kit is now available.


Monoclonal Antibody Acetylcholine Receptor Bone Marrow Sample Polyclonal Seron Predefined Specificity 


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  1. Dorfman, P. W. (ed.), 1982, GEN guide to biotechnology, Genet. Eng. News 2:6–19.Google Scholar
  2. Ephrussi, B., 1972, Hybridization of Somatic Cells, Princeton University Press, Princeton, New Jersey.Google Scholar
  3. Gerhard, W., Yewdell, J., Frankel, M. E., Lopes, A. D., and Staudt, L., 1980, Monoclonal antibodies against influenza virus, in: Monoclonal Antibodies. Hybridomas: A New Dimension in Biological Analyses (R. H. Kennett, T. J. McKearn, and K. B. Bechtol, eds.), Plenum Press, New York, pp. 317–333.Google Scholar
  4. Herlyn, D., and Koprowski, H., 1982, IgG2a monoclonal antibodies inhibit human tumor growth through interaction with effector cells, Proc. Natl. Acad. Sci. USA 79:4761–4765.CrossRefGoogle Scholar
  5. Jonak, Z. L., Kennett, R. H. and Bechtol, K. B., 1982, Detection of neuroblastoma cells in bone marrow using a combination of monoclonal antibodies, Hybridoma 1:349–368.CrossRefGoogle Scholar
  6. Kennett, R. H., 1979, Cell fusion, in: Methods in Enzymology, Volume LVIII (W. B. Jakoby and I. H. Pastan, eds.), Academic Press, New York, pp. 345–367.Google Scholar
  7. Kennett, R. H., and Gilbert, F., 1979, Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain, Science 203:1120–1123.CrossRefGoogle Scholar
  8. Kohler, G., and Milstein, C., 1975, Continuous culture of fused cells secreting antibody of predefined specificity, Nature 256:495–497.CrossRefGoogle Scholar
  9. Koprowski, H., and Wiktor, T., 1980, Monoclonal antibodies against rabies virus, in: Monoclonal Antibodies. Hybridomas: A New Dimension in Biological Analysis (R. H. Kennett, T. J. McKearn, and K. B. Bechtol, eds.), Plenum Press, New York, pp. 335–351.Google Scholar
  10. Lane, D., and Koprowski, H., 1982, Molecular recognition and the future of monoclonal antibodies, Nature 296:200–202.CrossRefGoogle Scholar
  11. Laver, W. G., Air, G. M., Webster, R. G., Gerhard, W., Ward, C. W., and Dopheide, T. A., 1979, Antigenic drift in a type A influenza virus sequence differences in the hemagglutinin of Hong Kong (H3N2) variants selected with monoclonal antibodies, Virology 98:226–237.CrossRefGoogle Scholar
  12. Lennon, V. A., and Lambert, E. H., 1981, Analysis of the antigenicity of acetylcholine receptor using monoclonal hybridoma antibodies, Ann. N. Y. Acad. Sci. 377:143–157.CrossRefGoogle Scholar
  13. Melchers, F., Potter, M., and Warner, N. L., 1978, Curr. Top. Microbiol. Immunol. 81.Google Scholar
  14. Mihovilovic, M., and Richman, D. P., 1982, Monoclonal antibody to alpha-bungarotoxin binding site of Torpedo acetylcholine receptor modified cholinergic ligand binding to solubilized and membrane-bound acetylcholine receptor, Soc. Neurosci. Abstr. 8:336.Google Scholar
  15. Potter, M., 1972, Immunoglobulin-producing tumors and myeloma proteins of mice, Physiol. Rev. 52:631–719.Google Scholar
  16. Richman, D. P., Gonez, C. M., Berman, P. W., Burres, S. A., Fitch, F. W., and Arnason, B. G. W., 1980, Monoclonal anti-acetylcholine receptor antibodies can cause experimental myesthenia, Nature 386:738–739.CrossRefGoogle Scholar
  17. Slaughter, C. A., Coseo, M. C., Abrams, C., Cancro, M. P., and Harris, H., 1980, The use of hybridomas in enzyme genetics, in: Monoclonal Antibodies. Hybrodomas: A New Dimension in Biological Analyses (R. H. Kennett, T.J. McKearn, and K. B. Bechtol, eds.), Plenum Press, New York, pp. 103–120.Google Scholar
  18. Tzartos, S. J., and Lindstrom, J. M., 1980, Monoclonal antibodies used to prove acetylcholine receptor structure: Localization of the main immunogenic regions and detection of similarities between subunits, Proc. Natl. Acad. Sci. USA 77:755–779.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Roger H. Kennett
    • 1
  • Kathleen B. Bechtol
    • 2
  • Thomas J. McKearn
    • 3
  1. 1.Department of Human GeneticsUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.The Wistar Institute of Anatomy and BiologyPhiladelphiaUSA
  3. 3.Cytogen CorporationPrinceton-Forrestal CenterPrincetonUSA

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