Cytogenetic Abnormalities as an Indicator of Mutagenic Exposure
Chromosome aberrations can essentially be studied in any cycling cell population, or in any cell population that can be stimulated by a mitogenic agent to cycle. In animals there are several cell types that fit these criteria, but for humans there are to all intents and purposes only two types that are practically suitable. These are the bone marrow cells, which are a cycling population, and peripheral lymphocytes, which are normally noncycling, but can be stimulated to enter the cell cycle by in vitro culturing with a mitogen such as phytohem-agglutinin. Because of ease of obtaining blood samples in contrast to taking bone marrow samples, the majority of studies on the induction of chromosome alterations in humans have been with the lymphocyte assay. This chapter will concentrate on this assay, with some additional comments on the bone marrow assav where appropriate.
KeywordsChromosome Aberration Cytogenetic Abnormality Peripheral Lymphocyte Bone Marrow Sample Ataxia Telangiectasia
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