Application of Antibodies to 5-Bromodeoxyuridine for the Detection of Cells of Rare Genotype
A current objective of research in the area of environmental mutagenesis is the development of automated methods of assessing in vivo and in vitro genetic damage.(1,2) The primary purpose of automating mutagen detection is to increase the speed and statistical reliability that is afforded by the instrumentation being recruited to this task, flow or image cytometry. In addition, this approach would eliminate the human bias and error characteristic of manual methods. In the case of in vivo assessment of mutagenesis, a method would be available to measure genetic variation in cells that either do not clone or clone at extremely low efficiencies.
KeywordsSister Chromatid Unlabeled Cell Rare Genotype Indirect Immunoperoxidase Technique Dansyl Hydrazine
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