Use of Fluorescence-Activated Cell Sorter for Screening Mutant Cells
Until recently, virtually all measurements of cellular mutation frequencies have been based on clonogenic assays. In fact, clonogenicity has been considered essential for the legitimate identification of mutant cells because it confirms the transmissbility of the variant phenotype. However, largely because of the apparent connection between somatic mutagenesis and carcinogenesis, it has become important to measure mutational injury in samples of normal somatic cells that generally have little or no replicative capacity. As a result, a number of efforts are underway to develop nonclonogenic mutation assays applicable to cells obtained from in vivo tissue samples and to find alternative means for authenticating the mutant pedigree of detected cells.
KeywordsMutant Cell Single Amino Acid Substitution Normal Hemoglobin Mutation Assay Human Hemoglobin
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